Streptozotocin-induced diabetes decreases placenta growth factor (PIGF) levels in rat placenta

• Published in: Journal of Veterinary Medical Science Vol. 69; no. 9; pp. 877 - 880
• Main Authors: Koh, P.O.(Gyeongsang National Univ., Jinju (Korea R.)), Sung, J.H, Won, C.K, Cho, J.H, Moon, J.G, Park, O.S, Kim, M.O
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.09.2007; Japan Science and Technology Agency
• Subjects: Animals; Blood Glucose - metabolism; Blotting, Western; Body Weight - physiology; DIABETE; DIABETES; Diabetes Mellitus, Experimental - blood; Diabetes Mellitus, Experimental - metabolism; Diabetes, Gestational - blood; Diabetes, Gestational - metabolism; FACTEUR DE CROISSANCE; FACTORES DE CRECIMIENTO; Female; Fetal Weight - physiology; Fetus; GESTACION; GESTATION; GROWTH FACTORS; Immunohistochemistry; Male; Organ Size - physiology; PLACENTA; Placenta - metabolism; Placenta Growth Factor; PlGF; PREGNANCY; Pregnancy Proteins - biosynthesis; Pregnancy Proteins - genetics; Pregnancy Proteins - metabolism; Random Allocation; RAT; RATA; RATS; Rats, Sprague-Dawley; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.69.877

The placenta produces several growth factors, including placenta growth factor (PIGF), which are essential for placenta growth and fetal growth. Diabetic pregnancy induces the abnormal placental growth and fetal development. This study investigated whether diabetes in pregnant rats induces changes in PIGF expression in the placenta. Diabetes was induced by a single intravenous injection of streptozotocin (35 mg/kg body weight) on day 0 of pregnancy, blood and tissue samples were collected on day 20 of pregnancy. In the diabetic group, maternal body weight and fetal weight significantly decreased compared to controls. RT-PCR and Western blot analyses showed that expression of PIGF was significantly decreased in placenta by streptozotocin treatment. Immunohistochemical study showed that the positive signal of PIGF in trophoblast cells was decreased in the diabetic group compared to controls. These findings demonstrate the decline of PIGF in the placenta in diabetic pregnancy.