Binding of the Virion Protein Mediating α Gene Induction in Herpes Simplex Virus 1-Infected Cells to Its Cis Site Requires Cellular Proteins

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 84; no. 20; pp. 7061 - 7065
• Main Authors: Jennifer L. C. Mc Knight, Kristie, Thomas M., Roizman, Bernard
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01.10.1987; National Acad Sciences
• Subjects: Antibodies; Binding Sites; Biological and medical sciences; Cell extracts; Cell nucleus; DNA; Fundamental and applied biological sciences. Psychology; Gels; Genes; HeLa cells; HeLa Cells - analysis; herpes simplex virus 1; Human herpesvirus 1; Messenger RNA; Molecular and cellular biology; Molecular genetics; Promoter Regions, Genetic; Protein Binding; Proteins - metabolism; Sequence Homology, Nucleic Acid; Simplexvirus - genetics; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; Viral Proteins - metabolism; Virion - analysis; Virions; Virus; Proteins; Regulation(control); Herpesvirus hominis 1; Transcription; Induction; Herpesviridae; DNA; Alphaherpesvirinae; Molecular interaction; Gene expression
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.84.20.7061

In herpes simplex virus 1-infected cells, the transcription of α genes, the first set of genes to be expressed, is induced by a virion component, the α -trans-induction factor, and requires a cis site. Homologs of the cis site are present in the promoter-regulatory domains of all α genes and bind two cellular proteins designated as α H1 and α H2-α H3. We report that α -trans-induction factor, synthesized in vitro or present in nuclear extracts of infected cells, forms complexes with viral DNA fragments containing its cis-acting site only in the presence of cellular proteins and only under conditions that also enable the binding of the α H1 protein to the DNA. The induction of α genes by α -trans-induction factor appears, therefore, to be mediated by the interaction of the viral protein with cellular proteins at its cis-acting site.