Protecting Effect of Phytoncide Solution, on Normal Human Dermal Fibroblasts against Reactive Oxygen Species

• Published in: Journal of Oleo Science Vol. 58; no. 8; pp. 429 - 436
• Main Authors: Fujimori, Hiroaki, Hisama, Masayoshi, Shibayama, Hiroharu, Iwaki, Masahiro
• Format: Journal Article
• Language: English
• Published: Japan Japan Oil Chemists' Society 2009; Japan Science and Technology Agency
• Subjects: collagen content; Dose-Response Relationship, Drug; Fibroblasts - drug effects; Fibroblasts - metabolism; Humans; Hydrogen Peroxide - pharmacology; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase Inhibitors; matrix metalloproteinase-1 (MMP-1); normal human dermal fibroblasts; oxidative stress; Oxidative Stress - drug effects; Phytoncide solution; Plant Extracts - pharmacology; Poaceae - chemistry; Reactive Oxygen Species - metabolism; Skin - cytology; Solutions; tert-Butylhydroperoxide - pharmacology; Ultraviolet Rays
• ISSN: 1345-8957; 1347-3352
• DOI: 10.5650/jos.58.429

Four types of phytoncide solutions (A-Type, AB-Type, D-Type and G-Type) was evaluated for reduction of cell damage induced by oxidative stress, ultraviolet A (UVA), ultraviolet B (UVB), hydroxyperoxide (H2O2) and t-butyl-hydroperoxide (t-BHP); stimulation of collagen synthesis against UVA irradiation; and inhibition of matrix metalloproteinase-1 (MMP-1) activity induced by UVA in human normal dermal fibroblasts and human reconstituted skin model. The A-Type, AB-Type, D-Type and G-Type of phytoncide solutions pretreatment resulted in significant protection against cell damage induced by UVB, UVA, H2O2 and t-BHP. The amount of type I collagen following UVA irradiation was increased by treatment with phytoncide solutions in a concentration-dependent manner. On the other hand, phytoncide solutions also suppressed the excess MMP-1 irradiated UVA in a concentration-dependent manner. These effects of G-type solution were superior to those of other types solutions.