What is the “modified” CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol

• Published in: Applications in plant sciences Vol. 11; no. 3; pp. e11517 - n/a
• Main Authors: Schenk, John J., Becklund, L. Ellie, Carey, S. James, Fabre, Paige P.
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.05.2023; John Wiley and Sons Inc; Wiley
• Subjects: cell lysis; cetyltrimethylammonium bromide; cetyltrimethylammonium bromide (CTAB); chemical composition; Deoxyribonucleic acid; DNA; DNA extraction; genomics; high‐throughput sequencing; Metabolites; molecular biology; molecular systematics; Oxidation; Phylogenetics; plant genetics; Plant sciences; Protocol; Review; Secondary metabolites; molecular biology; DNA extraction; high‐throughput sequencing; cetyltrimethylammonium bromide (CTAB); molecular systematics; cell lysis; plant genetics

Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization. Research articles often cite a “modified” CTAB protocol without explicitly stating ho...