What is the “modified” CTAB protocol? Characterizing modifications to the CTAB DNA extraction protocol

Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization. Research articles often cite a “modified” CTAB protocol without explicitly stating ho...

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Bibliographic Details
Published inApplications in plant sciences Vol. 11; no. 3; pp. e11517 - n/a
Main Authors Schenk, John J., Becklund, L. Ellie, Carey, S. James, Fabre, Paige P.
Format Journal Article
LanguageEnglish
Published United States John Wiley & Sons, Inc 01.05.2023
John Wiley and Sons Inc
Wiley
Subjects
cell lysis
cetyltrimethylammonium bromide
cetyltrimethylammonium bromide (CTAB)
chemical composition
Deoxyribonucleic acid
DNA
DNA extraction
genomics
high‐throughput sequencing
Metabolites
molecular biology
molecular systematics
Oxidation
Phylogenetics
plant genetics
Plant sciences
Protocol
Review
Secondary metabolites
molecular biology
DNA extraction
high‐throughput sequencing
cetyltrimethylammonium bromide (CTAB)
molecular systematics
cell lysis
plant genetics
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Summary:Cetyltrimethylammonium bromide (CTAB)–based methods are widely used to isolate DNA from plant tissues, but the unique chemical composition of secondary metabolites among plant species has necessitated optimization. Research articles often cite a “modified” CTAB protocol without explicitly stating how the protocol had been altered, creating non‐reproducible studies. Furthermore, the various modifications that have been applied to the CTAB protocol have not been rigorously reviewed and doing so could reveal optimization strategies across study systems. We surveyed the literature for modified CTAB protocols used for the isolation of plant DNA. We found that every stage of the CTAB protocol has been modified, and we summarized those modifications to provide recommendations for extraction optimization. Future genomic studies will rely on optimized CTAB protocols. Our review of the modifications that have been used, as well as the protocols we provide here, could better standardize DNA extractions, allowing for repeatable and transparent studies.
Bibliography:This article is part of the special issue “Emerging Methods in Botanical DNA/RNA Extraction.”
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ISSN:2168-0450
2168-0450
DOI:10.1002/aps3.11517