Gene Regulation by Intracellular Delivery and Photodegradation of Nanoparticles Containing Small Interfering RNA

• Published in: Macromolecular bioscience Vol. 14; no. 5; pp. 626 - 631
• Main Authors: Murayama, Shuhei, Kos, Petra, Miyata, Kanjiro, Kataoka, Kazunori, Wagner, Ernst, Kato, Masaru
• Format: Journal Article
• Language: English
• Published: Germany Blackwell Publishing Ltd 01.05.2014; Wiley Subscription Services, Inc
• Subjects: Base Sequence; Carriers; Cell Line, Tumor; Drug Delivery Systems - methods; Electrophoresis, Agar Gel; Electrophoretic Mobility Shift Assay; Encapsulation; gene delivery; Gene expression; Gene Expression Regulation - drug effects; Gene Expression Regulation - genetics; Genes; Humans; Irradiation; Molecular Sequence Data; Molecular Structure; nanoparticle; Nanoparticles; Nanoparticles - metabolism; photodegradable; Photodegradation; photoinduced; Photolysis; Ribonucleic acids; RNA, Small Interfering - metabolism; RNA, Small Interfering - pharmacology; siRNA; spatiotemporal regulation; Ultraviolet radiation; gene delivery; nanoparticle; siRNA; photoinduced; spatiotemporal regulation; photodegradable
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201300393

Although the use of small interfering RNA (siRNA) is a promising technique for gene regulation, spatiotemporal control of the effects of siRNA must be achieved if the technique is to be safe and practical. Here, a method for spatiotemporal regulation of genes with nanoparticles containing siRNA is reported. The siRNA is encapsulated in photodegradable nanoparticles that are internalized to SKOV3‐Luc cells, where the siRNA is released from the nanoparticles by UV irradiation for 30 s. The encapsulated siRNA only shows no gene‐silencing effects, but release of the siRNA upon UV radiation leads to sequence‐specific silencing of the luciferase gene in the cells. These results indicate that photodegradable siRNA‐containing nanoparticles can be useful for time‐ and space‐dependent regulation of gene expression in cells. Photodegradable nanoparticle is proposed as the siRNA carrier to regulate genes in cells. Without UV, the siRNA shows no gene‐silencing effects, after UV irradiation, siRNA is released leading to sequence‐specific silencing of the mRNA. This indicates that siRNA‐containing nanoparticles can be useful for spatiotemporal regulation of gene expression in cells.