电化学和紫外-可见吸收光谱法研究pH和金纳米粒子对细胞色素c的影响
制备了粒径均匀、平均粒子尺度为（4.7±0.6）nm,表面修饰3-巯基丙酸（MPA）的金纳米粒子（AuNPs）.利用电化学和紫外-可见（UV-Vis）吸收光谱研究了pH和AuNPs对细胞色素c（Cytc）结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cytc和Cytc-AuNPs复合物的结构没有发生明显变化.当pH=2.0时,Cytc和Cytc-AuNPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安（CV）结果表明,表面修饰了MPA的AuNPs能促进Cytc和电极之间的电子传输,与修饰了柠檬酸根的AuNPs相比,其生物兼容性更好.pH的变化...
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| Published in | 物理化学学报 Vol. 28; no. 5; pp. 1127 - 1133 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
厦门大学化学化工学院化学系,固体表面物理化学国家重点实验室,福建厦门361005%香港城市大学物理与材料科学系,香港
2012
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| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-6818 |
| DOI | 10.3866/PKU.WHXB201203073 |
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| Abstract | 制备了粒径均匀、平均粒子尺度为（4.7±0.6）nm,表面修饰3-巯基丙酸（MPA）的金纳米粒子（AuNPs）.利用电化学和紫外-可见（UV-Vis）吸收光谱研究了pH和AuNPs对细胞色素c（Cytc）结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cytc和Cytc-AuNPs复合物的结构没有发生明显变化.当pH=2.0时,Cytc和Cytc-AuNPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安（CV）结果表明,表面修饰了MPA的AuNPs能促进Cytc和电极之间的电子传输,与修饰了柠檬酸根的AuNPs相比,其生物兼容性更好.pH的变化会引起CV中Cytc峰电流的改变和峰电位的移动.随着pH值的降低,Cytc电活性的量逐渐减小,并且pH诱导Cytc发生不可逆变性.AuNPs的引入使自由态的Cytc耐酸性增强,而使得吸附态的Cytc耐酸能力减弱. |
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| AbstractList | 制备了粒径均匀、平均粒子尺度为（4.7±0.6）nm,表面修饰3-巯基丙酸（MPA）的金纳米粒子（AuNPs）.利用电化学和紫外-可见（UV-Vis）吸收光谱研究了pH和AuNPs对细胞色素c（Cytc）结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cytc和Cytc-AuNPs复合物的结构没有发生明显变化.当pH=2.0时,Cytc和Cytc-AuNPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安（CV）结果表明,表面修饰了MPA的AuNPs能促进Cytc和电极之间的电子传输,与修饰了柠檬酸根的AuNPs相比,其生物兼容性更好.pH的变化会引起CV中Cytc峰电流的改变和峰电位的移动.随着pH值的降低,Cytc电活性的量逐渐减小,并且pH诱导Cytc发生不可逆变性.AuNPs的引入使自由态的Cytc耐酸性增强,而使得吸附态的Cytc耐酸能力减弱. O646; 制备了粒径均匀、平均粒子尺度为(4.7±0.6) nm,表面修饰3-巯基丙酸(MPA)的金纳米粒子(Au NPs).利用电化学和紫外-可见(UV-Vis)吸收光谱研究了pH和Au NPs对细胞色素c(Cytc)结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cyt c和Cyt c-Au NPs复合物的结构没有发生明显变化.当pH=2.0时,Cyt c和Cyt c-Au NPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安(CV)结果表明,表面修饰了MPA的Au NPs能促进Cyt c和电极之间的电子传输,与修饰了柠檬酸根的Au NPs相比,其生物兼容性更好.pH的变化会引起CV中Cytc峰电流的改变和峰电位的移动.随着pH值的降低,Cytc电活性的量逐渐减小,并且pH诱导Cyt c发生不可逆变性.Au NPs的引入使自由态的Cytc耐酸性增强,而使得吸附态的Cytc耐酸能力减弱. |
| Author | 王艳艳 姜艳霞 沙德礼 罗德礼 孙世刚 |
| AuthorAffiliation | 厦门大学化学化工学院化学系,固体表面物理化学国家重点实验室,福建厦门361005 香港城市大学物理与材料科学系,香港 |
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| Author_FL | SUN Shi-Gang ROGACH Andrey JIANG Yan-Xia SUSHAAndrei WANG Yan-Yan |
| Author_FL_xml | – sequence: 1 fullname: WANG Yan-Yan – sequence: 2 fullname: JIANG Yan-Xia – sequence: 3 fullname: SUSHAAndrei – sequence: 4 fullname: ROGACH Andrey – sequence: 5 fullname: SUN Shi-Gang |
| Author_xml | – sequence: 1 fullname: 王艳艳 姜艳霞 沙德礼 罗德礼 孙世刚 |
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| ClassificationCodes | O646 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DOI | 10.3866/PKU.WHXB201203073 |
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| DocumentTitleAlternate | Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy |
| DocumentTitle_FL | Effect of pH and Au Nanoparticles on Cytochrome c Investigated by Electrochemistry and UV-Vis Absorption Spectroscopy |
| EndPage | 1133 |
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| Issue | 5 |
| Keywords | 循环伏安法 紫外-可见吸收光谱 细胞色素c 金纳米粒子 pH值 |
| Language | Chinese |
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| Notes | Au nanoparticle; Cytochrome c; pH value; Cyclic voltammetry; UV-Vis absorption spectroscopy 11-1892/06 WANG Yan-Yan JIANG Yan-Xia , SUSHA Andrei ROGACH Andrey SUN Shi-Gang （1 Key Laboratory of Physical Chemistry of Solid Surface,Department of Chemistry,College of Chemistry and Chemistry Engineering,Xiamen University,Xiamen 361005,Fujian Province,P.R.China;2 Department of Physics and Materials Science,City University of Hong Kong,Hong Kong,P.R.China） In this paper,gold nanoparticles（NPs）with an average size of（4.7±0.6）nm,capped with mercaptopropionic acid（MPA）ligand,are prepared.The effect of pH and Au NPs on cytochrome c（Cyt c） is investigated by electrochemistry and UV-Vis absorption spectroscopy.UV-Vis absorption spectra indicate that the structures of Cyt c and Cyt c-Au NPs complex do not change appreciably between pH=7.5 and pH=3.0,but their Soret band positions change markedly at pH=2.0,indicating that low pH value induces a conformational change in Cyt c.Cyclic voltammetry（CV）result shows that Au NPs cappe |
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| PublicationTitle | 物理化学学报 |
| PublicationTitleAlternate | Acta Physico-Chimica Sinica |
| PublicationTitle_FL | Acta Physico-Chimica Sinica |
| PublicationYear | 2012 |
| Publisher | 厦门大学化学化工学院化学系,固体表面物理化学国家重点实验室,福建厦门361005%香港城市大学物理与材料科学系,香港 |
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| Snippet | ... O646; 制备了粒径均匀、平均粒子尺度为(4.7±0.6) nm,表面修饰3-巯基丙酸(MPA)的金纳米粒子(Au NPs).利用电化学和紫外-可见(UV-Vis)吸收光谱研究了pH和Au NPs对细胞色素c(Cytc)结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cyt c和Cyt c-Au... |
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| SubjectTerms | pH值 循环伏安法 紫外-可见吸收光谱 细胞色素c 金纳米粒子 |
| Title | 电化学和紫外-可见吸收光谱法研究pH和金纳米粒子对细胞色素c的影响 |
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