电化学和紫外-可见吸收光谱法研究pH和金纳米粒子对细胞色素c的影响

制备了粒径均匀、平均粒子尺度为（4.7±0.6）nm,表面修饰3-巯基丙酸（MPA）的金纳米粒子（AuNPs）.利用电化学和紫外-可见（UV-Vis）吸收光谱研究了pH和AuNPs对细胞色素c（Cytc）结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cytc和Cytc-AuNPs复合物的结构没有发生明显变化.当pH=2.0时,Cytc和Cytc-AuNPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安（CV）结果表明,表面修饰了MPA的AuNPs能促进Cytc和电极之间的电子传输,与修饰了柠檬酸根的AuNPs相比,其生物兼容性更好.pH的变化...

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Bibliographic Details
Published in物理化学学报 Vol. 28; no. 5; pp. 1127 - 1133
Main Author 王艳艳 姜艳霞 沙德礼 罗德礼 孙世刚
Format Journal Article
LanguageChinese
Published 厦门大学化学化工学院化学系,固体表面物理化学国家重点实验室,福建厦门361005%香港城市大学物理与材料科学系,香港 2012
Subjects
pH值
循环伏安法
紫外-可见吸收光谱
细胞色素c
金纳米粒子
循环伏安法
紫外-可见吸收光谱
细胞色素c
金纳米粒子
pH值
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ISSN1000-6818
DOI10.3866/PKU.WHXB201203073

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Summary:制备了粒径均匀、平均粒子尺度为（4.7±0.6）nm,表面修饰3-巯基丙酸（MPA）的金纳米粒子（AuNPs）.利用电化学和紫外-可见（UV-Vis）吸收光谱研究了pH和AuNPs对细胞色素c（Cytc）结构的影响.UV-Vis吸收光谱结果表明,pH为7.5-3.0时,Cytc和Cytc-AuNPs复合物的结构没有发生明显变化.当pH=2.0时,Cytc和Cytc-AuNPs复合物的Soret谱峰位置均发生明显移动,说明pH诱导其构象发生变化.循环伏安（CV）结果表明,表面修饰了MPA的AuNPs能促进Cytc和电极之间的电子传输,与修饰了柠檬酸根的AuNPs相比,其生物兼容性更好.pH的变化会引起CV中Cytc峰电流的改变和峰电位的移动.随着pH值的降低,Cytc电活性的量逐渐减小,并且pH诱导Cytc发生不可逆变性.AuNPs的引入使自由态的Cytc耐酸性增强,而使得吸附态的Cytc耐酸能力减弱.
Bibliography:Au nanoparticle; Cytochrome c; pH value; Cyclic voltammetry; UV-Vis absorption spectroscopy
11-1892/06
WANG Yan-Yan JIANG Yan-Xia , SUSHA Andrei ROGACH Andrey SUN Shi-Gang （1 Key Laboratory of Physical Chemistry of Solid Surface,Department of Chemistry,College of Chemistry and Chemistry Engineering,Xiamen University,Xiamen 361005,Fujian Province,P.R.China;2 Department of Physics and Materials Science,City University of Hong Kong,Hong Kong,P.R.China）
In this paper,gold nanoparticles（NPs）with an average size of（4.7±0.6）nm,capped with mercaptopropionic acid（MPA）ligand,are prepared.The effect of pH and Au NPs on cytochrome c（Cyt c） is investigated by electrochemistry and UV-Vis absorption spectroscopy.UV-Vis absorption spectra indicate that the structures of Cyt c and Cyt c-Au NPs complex do not change appreciably between pH=7.5 and pH=3.0,but their Soret band positions change markedly at pH=2.0,indicating that low pH value induces a conformational change in Cyt c.Cyclic voltammetry（CV）result shows that Au NPs cappe
ISSN:1000-6818
DOI:10.3866/PKU.WHXB201203073