多种肿瘤标志物在肺腺癌中的表达及预后意义的研究

背景与目的肺腺癌是最常见的肺癌组织学类型，发病率呈上升趋势。本研究旨在探讨NapsinA、TTF-1、ERCC1、RRM1、EGFR、HER2、ERa、ERB、PR、Bcl-2蛋白在肺腺癌中的表达及与临床病理特征和预后的关系。方法将227例肺腺癌石蜡标本构建组织芯片。采用免疫组化方法检测10种靶蛋白标记物在肺腺癌中的表达，分析各蛋白表达与患者临床病理特征和预后的关系。结果10种蛋白中仅NapsinA蛋白表达与性别有关（P=0．049）；Napsin A、PR和EGFR蛋白表达与吸烟有关，TTF-1和ERCC1蛋白表达与肿瘤大小有关，NapsinA、TTF-1、ERα和PR蛋白表达与肿瘤分化程度...

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Bibliographic Details
Published in中国肺癌杂志 Vol. 17; no. 3; pp. 243 - 253
Main Author 杨欣 薛丽燕 郭蕾 温芃 林冬梅
Format Journal Article
LanguageChinese
Published 北京协和医学院中国医学科学院肿瘤医院病理科,北京,100021 2014
Subjects
免疫组化
组织芯片
肺肿瘤
预后
Immunohistochemistry
Prognosis
组织芯片
预后
Tissue microarray
肺肿瘤
免疫组化
Lung neoplasms
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ISSN1009-3419
1999-6187
DOI10.3779/j.issn.1009-3419.2014.03.11

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Summary:背景与目的肺腺癌是最常见的肺癌组织学类型，发病率呈上升趋势。本研究旨在探讨NapsinA、TTF-1、ERCC1、RRM1、EGFR、HER2、ERa、ERB、PR、Bcl-2蛋白在肺腺癌中的表达及与临床病理特征和预后的关系。方法将227例肺腺癌石蜡标本构建组织芯片。采用免疫组化方法检测10种靶蛋白标记物在肺腺癌中的表达，分析各蛋白表达与患者临床病理特征和预后的关系。结果10种蛋白中仅NapsinA蛋白表达与性别有关（P=0．049）；Napsin A、PR和EGFR蛋白表达与吸烟有关，TTF-1和ERCC1蛋白表达与肿瘤大小有关，NapsinA、TTF-1、ERα和PR蛋白表达与肿瘤分化程度有关，TTF-1、Bcl-2和ERCC1蛋白表达与病理分期有关（P〈0．05）。10种蛋白表达均与年龄无关，ERβ、HER2和RRM1蛋白表达与各项临床病理参数均无相关性（P〉0．05）。单因素生存分析显示，NapsinA、TTF．1和ERCC1蛋白表达与患者总生存相关；TTF-1蛋白表达与患者无病生存相关（P〈0．05）。进一步分析I期肺腺癌，仅NapsinA蛋白表达与患者总生存相关，P〈0．05；10种蛋白表达均与I期患者无病生存无关（P〉0．05）。经多因素生存分析，仅病理分期与患者总生存和无病生存相关（P〈0．05）；10种蛋白表达均未显示与患者生存的相关（P〉0．05）。结论Napsin A、TrF-1和ERCC1蛋白可能是提示肺腺癌患者预后较好的标记物。
Bibliography:Tissue microarray; Immunohistochemistry; Lung neoplasms; Prognosis
Xin YANG, Liyan XUE, Lei GUO, Peng WEN, （Dongmei LIN Department of Pathology, Cancer Institute （Hospital）, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100021j China）
12-1395/R
Background and objective Lung adenocarcinoma is one of the most common histological subtypes of lung cancer. The incidence of this disease was continuously increased. This study aims to detect the expressions of Napsin A, TTF- 1, ERCC1, RRM1, EGFR, HER2, ERa, ERβ, PR, and Bcl-2 in lung adenocarcinoma and to explore their correlations with clinicopathological characteristics and prognosis. Methods A total of 227 lung adenocarcinoma specimens were constructed in tissue microarrays. The expressions of the 10 tumor biomarkers were analyzed by immunohistochemistry on paraffinembedded sections. Results Among the 10 markers, Napsin A was gender-related （P=0.049）. Napsin A, PR, and EGFR were significantly associated with smoking. TTF-1 and ERCC1 were
ISSN:1009-3419
1999-6187
DOI:10.3779/j.issn.1009-3419.2014.03.11