茶树组织培养中外植体褐化控制的研究

为了控制茶树外植体在组织培养过程中的褐化现象,从外植体表面消毒时间、抗氧化剂和吸附剂的使用、琼脂浓度和pH值的高低进行了综合性比较试验。结果表明：外植体表面消毒时间以6 min为宜;外植体接种在MS＋6-BA 2.0 mg/L＋NAA 0.2mg/L＋GA31 mg/L＋核黄素0.02 g/L的培养基上,琼脂浓度为5.0 g/L,pH值为5.6,外植体生长状况良好,褐化率由57%下降为13.3%。...

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Published in西南农业学报 Vol. 25; no. 3; pp. 1065 - 1068
Main Author 邬秀宏 李中林 陈正明 朱学栋 邓敏 张莹 王延华 唐敏
Format Journal Article
LanguageChinese
Published 重庆市农业科学院茶叶研究所,重庆市茶叶工程技术研究中心,重庆永川402160%重庆市农业科学院农业工程研究所,重庆,401329%重庆市永川区经济作物技术推广站,重庆永川,402160 2012
Subjects
外植体
茶树
褐化
褐化
外植体
茶树
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ISSN1001-4829
DOI10.3969/j.issn.1001-4829.2012.03.067

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Summary:为了控制茶树外植体在组织培养过程中的褐化现象,从外植体表面消毒时间、抗氧化剂和吸附剂的使用、琼脂浓度和pH值的高低进行了综合性比较试验。结果表明：外植体表面消毒时间以6 min为宜;外植体接种在MS＋6-BA 2.0 mg/L＋NAA 0.2mg/L＋GA31 mg/L＋核黄素0.02 g/L的培养基上,琼脂浓度为5.0 g/L,pH值为5.6,外植体生长状况良好,褐化率由57%下降为13.3%。
Bibliography:Tea trees; Explants; Browning
51-1213/S
WU Xiu-hong , LI Zhong-lin ,CHEN Zheng-ming, ZHU Xue-dong ,DENG Min,ZHANG Ying, WANG Ting-hua ,TANG Min （ 1. Institute of Tea Research, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China ;2. Institute of Agricultural Engineer- ing, Chongqing Academy of Agricultural Science, Chongqing 401329, China;3. Economic Crop Technology Promotion Station of Yongchnan Chongqing, Yongchuan Chongqing 402160, China ）
The sterilized time of the tea explants, different antioxidants and adsorbents used, and different concentration ofpH and agar were studied to reduce the browning rate of explants in tissue culture of tea tree. The results showed that the browning rate would decrease 57 % to 13.3 % when the surface disinfection suitable time lasted about 6min,and explants were inoculated on MS ＋ 6-BA 2. 0 mg/L ＋ NAA 0. 2 mg/L ＋ GA3 lmg/L ＋ VB2 0. 02 g/L of medium, with agar concentration to 5. 0 g/L, pH of 5.6, and the growth of exphmts was in good condition.
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.03.067