金黄色葡萄球菌CP5偶联鞭毛蛋白疫苗对小鼠乳腺炎模型的保护作用

以鞭毛蛋白（flagellin）作为载体蛋白，通过化学偶联至金黄色葡萄球菌5型荚膜多糖（CP5），并在小鼠模型上检验多糖疫苗对小鼠存活及其乳腺的保护作用。将小鼠分为4组：生理盐水组、CP5单独免疫组、牛血清白蛋白（BSA）-CP5偶联组和nagellin—CP5偶联组，每组10只，分别进行皮下免疫，均含CP510μg；共免疫两次，两次免疫间隔3周。第2次免疫两周后，用金黄色葡萄球菌分别对小鼠腹腔、乳腺进行攻毒，并通过实时定量PCR对乳腺组织细胞因子IL-4、IL-10、IFN-γ、IL-12的表达进行检测。结果表明，CP5偶联鞭毛蛋白疫苗对小鼠乳腺具有显著的保护效果，并在乳腺组织部位诱导了Th...

Full description

Saved in:
Bibliographic Details
Published in广东农业科学 Vol. 40; no. 13; pp. 120 - 122
Main Author 袁林 郭建军 王贤卓
Format Journal Article
LanguageChinese
Published 江西省科学院微生物研究所,江西南昌,330096%南昌大学生命科学与食品工程学院,江西南昌,330031 2013
Subjects
5型荚膜多糖
乳腺
细胞因子
鞭毛蛋白
鞭毛蛋白
乳腺
细胞因子
5型荚膜多糖
Online AccessGet full text
ISSN1004-874X

Cover

More Information
Summary:以鞭毛蛋白（flagellin）作为载体蛋白，通过化学偶联至金黄色葡萄球菌5型荚膜多糖（CP5），并在小鼠模型上检验多糖疫苗对小鼠存活及其乳腺的保护作用。将小鼠分为4组：生理盐水组、CP5单独免疫组、牛血清白蛋白（BSA）-CP5偶联组和nagellin—CP5偶联组，每组10只，分别进行皮下免疫，均含CP510μg；共免疫两次，两次免疫间隔3周。第2次免疫两周后，用金黄色葡萄球菌分别对小鼠腹腔、乳腺进行攻毒，并通过实时定量PCR对乳腺组织细胞因子IL-4、IL-10、IFN-γ、IL-12的表达进行检测。结果表明，CP5偶联鞭毛蛋白疫苗对小鼠乳腺具有显著的保护效果，并在乳腺组织部位诱导了Th1型细胞因子表达，说明鞭毛蛋白是CP5的理想载体蛋白。
Bibliography:44-1267/S
Taking flagellin as carrier protein, by chemical coupling to Staphylococcus aureus type 5 capsular polysaccharides, we tested the protective effect of polysaccharide vaccine on mouse model for breast. Mice were divided to 4 groups with 10 mice in each, they were immunized with saline, 10 μg CP5 alone, 10 μg CP5 conjugated with BSA （BSA-CP5）, 10 μg CP5 conjugated with flagellin （flagellin-CPS） respectively on the first day and the 21th day. Two weeks after the second immunization, mice were challenged with S.aureus in peritoneal or breast, and cytokines expression of IL-4, IL-10, IFN- γ, IL-12 in breast tissue were quantified by real-time PCR. The results showed that the CP5 conjugated with flagellin vaccine had a protective effect on mice breast, and induced Thl cytokines expression in breast tissue, suggestting that flagellin was an ideal carrier protein for CPS.
type 5 tcapsular polysaccharide; flagellin; breast; cytokines
YUAN Lin, GUO Jian-jun, WANG Xian-zhuo （1.Institute of Microbiology, Jiangxi A
ISSN:1004-874X