AtMYB50 regulates root cell elongation by upregulating PECTIN METHYLESTERASE INHIBITOR 8 in Arabidopsis thaliana

• Published in: PloS one Vol. 18; no. 12; p. e0285241
• Main Authors: Mase, Kosuke, Mizuno, Honomi, Nakamichi, Norihito, Suzuki, Takamasa, Kojima, Takaaki, Kamiya, Sho, Takeuchi, Taiga, Kondo, Chiko, Yamashita, Harumi, Sakaoka, Satomi, Morikami, Atsushi, Tsukagoshi, Hironaka
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.12.2023; Public Library of Science (PLoS)
• Subjects: Analysis; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Cell cycle; Cell differentiation; Cell growth; Cell Proliferation; Cellular signal transduction; Cloning; Cytokinins; Data analysis; Elongation; Fluorescence; Gene Expression Regulation, Plant; Gene regulation; Genes; Genetic transcription; Genomes; Genomics; Gibberellins; Growth; Homeostasis; Hydrogen peroxide; Indoleacetic Acids - metabolism; Inhibitors; Molecular modelling; Pectin; Pectinesterase; Peroxidase; Plant growth; Plant roots; Plant Roots - metabolism; Plasmids; Reactive oxygen species; Reactive Oxygen Species - metabolism; RNA; Root development; Roots (Botany); Seeds; Signal transduction; Software; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Japan; Germany; United Kingdom > UK; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0285241

Plant root development involves multiple signal transduction pathways. Notably, phytohormones like auxin and cytokinin are well characterized for their molecular mechanisms of action. Reactive oxygen species (ROS) serve as crucial signaling molecules in controlling root development. The transcription factor, UPBEAT1 (UPB1) is responsible for maintaining ROS homeostasis at the root tip, influencing the transition from cell proliferation to differentiation. While UPB1 directly regulates peroxidase expression to control ROS homeostasis, it targets genes other than peroxidases, suggesting its involvement in root growth through non-ROS signals. Our investigation focused on the transcription factor MYB50, a direct target of UPB1, in Arabidopsis thaliana. By analyzing multiple fluorescent proteins and conducting RNA-seq and ChIP-seq, we unraveled a step in the MYB50 regulatory gene network. This analysis, in conjunction with the UPB1 regulatory network, demonstrated that MYB50 directly regulates the expression of PECTIN METHYLESTERASE INHIBITOR 8 (PMEI8). Overexpressing PMEI8, similar to the MYB50, resulted in reduced mature cell length. These findings establish MYB50 as a regulator of root growth within the UPB1 gene regulatory network. Our study presents a model involving transcriptional regulation by MYB50 in the UPB1 regulated root growth system and sheds light on cell elongation via pectin modification.