Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers

• Published in: PloS one Vol. 19; no. 8; p. e0301284
• Main Authors: Antao, Noelle V., Sall, Joseph, Petzold, Christopher, Ekiert, Damian C., Bhabha, Gira, Liang, Feng-Xia
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.08.2024; Public Library of Science (PLoS)
• Subjects: Animals; Biometry; Carbon; Cell culture; Cells; Data collection; Data entry; Electron microscopy; Electrons; Embedding; Face; Humans; In vitro methods and tests; Ion beams; Light microscopy; Lipids; Microscopy, Electron, Scanning - methods; Monolayers; Optical microscopy; Parameter identification; Proteins; Sample preparation; Scanning electron microscopy; Specimen Handling - methods; Stains & staining; Tissue culture; Tissue Embedding - methods; Tissues; Ultrastructure; Volume Electron Microscopy; Workflow

Volume electron microscopy encompasses a set of electron microscopy techniques that can be used to examine the ultrastructure of biological tissues and cells in three dimensions. Two block face techniques, focused ion beam scanning electron microscopy (FIB-SEM) and serial block face scanning electro...