A Beta-Glucosidase Gene Downstream of the Cellulose Synthase Operon in Cellulose-producing Acetobacter

• Published in: Bioscience, biotechnology, and biochemistry Vol. 61; no. 10; pp. 1789 - 1790
• Main Authors: Tonouchi, Naoto, Tahara, Naoki, Kojima, Yukiko, Nakai, Tomonori, Sakai, Fukumi, Hayashi, Takahisa, Tsuchida, Takayasu, Yoshinaga, Fumihiro
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1997; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: Acetobacter; Acetobacter - enzymology; Acetobacter - genetics; Amino Acid Sequence; Bacteriology; Base Sequence; beta-glucosidase; beta-Glucosidase - genetics; Biological and medical sciences; Biology of microorganisms of confirmed or potential industrial interest; Biotechnology; Cellulose - biosynthesis; cellulose synthesis; Culture Media; Fundamental and applied biological sciences. Psychology; gene; Gene Expression Regulation, Enzymologic; Genes, Bacterial - genetics; Genetics; Microbiology; Mission oriented research; Molecular Sequence Data; Multigene Family; Open Reading Frames; Operon - genetics; Acetobacteraceae; Gene cluster; Nucleotide sequence; Enzyme; Cellulose; Biosynthesis; Acetobacter; β-Glucosidase; Glycosidases; Bacteria; Hydrolases; O-Glycosidases; Aminoacid sequence
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.61.1789

An open reading frame was found 214 bp downstream of the cellulose synthase operon of Acetobacter. The encoded amino acid sequence was found to be similar to some beta-glucosidases (G3ases). We detected G3ase activity in the culture medium and analysis of the N-terminal amino acid sequence showed that this gene encodes the enzyme. Therefore, it is possible that this region is a gene cluster for cellulose synthesis.