Cloning and expression of a complementary DNA encoding a high affinity human neurotensin receptor

• Published in: FEBS letters Vol. 317; no. 1; pp. 139 - 142
• Main Authors: Vita, N., Laurent, P., Lefort, S., Chalon, P., Dumont, X., Kaghad, M., Gully, D., Le Fur, G., Ferrara, P., Caput, D.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 08.02.1993; Elsevier
• Subjects: Amino Acid Sequence; Base Sequence; Binding; Binding, Competitive; Biological and medical sciences; Cell Line; Cloning; Cloning, Molecular; DNA; Fundamental and applied biological sciences. Psychology; Genes. Genome; HT 29 cell; Humans; Leukocytes, Mononuclear - metabolism; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Neurotensin; Neurotensin - metabolism; Receptor; Receptors, Neurotensin; Receptors, Neurotransmitter - genetics; Receptors, Neurotransmitter - metabolism; RNA, Messenger - metabolism; Sequence Homology, Amino Acid; Tumor Cells, Cultured; Binding; HT 29 cell; Neurotensin; Receptor; Cloning; Adenocarcinoma; Human; Ligand binding; Nucleotide sequence; Peptide hormone; Gene expression; Neuropeptide; Tissue; Cell line; Complementary DNA; Colon; Hormonal receptor
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)81509-X

A human neurotensin receptor (hNTR) cDNA was cloned from the colonic adenocarcinoma cell line HT29. The cloned cDNA encodes a putative peptide of 418 amino acids with 7 transmembrane domains. The amino acid sequence of the hNTR is 84% identical to the rat NTR [Neuron, 4 (1990) 847-854]. Transfection of this cDNA into COS cells results in the expression of receptors with pharmacological properties similar to those found with HT29 cells. Northern blot analysis using the hNTR cDNA probe indicated a single transcript of 4 kb in the brain, the small intestine and blood mononuclear cells.