超声波辅助酶促猪油甘油解制备甘油单酯的研究
以猪油和丙三醇为原料,采用超声辅助酶促甘油解反应合成甘油单酯,通过单因素试验及正交试验,确定其最佳酶促合成条件。结果表明,最佳反应条件:叔戊醇为溶剂,Novozym435用量10%(猪油质量分数),反应温度45℃,甘油与猪油物质的量的比4:I,反应时间6h,超声波频率28kHz,超声功率250w。在此条件下,甘油单酯产率达到(84.76±1.96)%。超声辅助酶促反应,不仅可以提高产物产率,还可缩短反应时间。...
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Published in | 江西农业学报 Vol. 25; no. 12; pp. 101 - 105 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
南京农业大学 食品科技学院,江苏 南京,210095
2013
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Subjects | |
Online Access | Get full text |
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Summary: | 以猪油和丙三醇为原料,采用超声辅助酶促甘油解反应合成甘油单酯,通过单因素试验及正交试验,确定其最佳酶促合成条件。结果表明,最佳反应条件:叔戊醇为溶剂,Novozym435用量10%(猪油质量分数),反应温度45℃,甘油与猪油物质的量的比4:I,反应时间6h,超声波频率28kHz,超声功率250w。在此条件下,甘油单酯产率达到(84.76±1.96)%。超声辅助酶促反应,不仅可以提高产物产率,还可缩短反应时间。 |
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Bibliography: | ZHAO Zhe, ZHANG Dan - ni, GAO Chun - yang, XIA Yuan - fang, WEI Wei, ZHAO Hal - zhen , LU Zhao - xin ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China) 36-1124/S Monoglyceride was synthesized by lipase -catalyzed glycerolysis of lard with glycerol through ultrasound assistance. Single - factor experiment and orthogonal test were conducted to optimize the glycerolysis conditions of lard. The optimum conditions for monoglyceride synthesis were acquired as follows: tert - amyl alcohol as reaction medium, Novozym 435 lipase dosage 10% ( calculated on the basis of the mass of lard), reaction temperature 45 ~C, molar ratio of glycerol to lard 4: 1, reaction time 6 h, ul- trasonic frequency 28 kHz, and ultrasonic power 250 W. Under these optimum conditions, the productive rate of monoglyceride was up to 84.76% -+ 1.96%. The ultrasound - assisted lipase - catalyzed treatment could not only improve the yield of product, but also shorten the reaction time. Lipase ; Monoglycer |
ISSN: | 1001-8581 |