超声波辅助酶促猪油甘油解制备甘油单酯的研究

以猪油和丙三醇为原料，采用超声辅助酶促甘油解反应合成甘油单酯，通过单因素试验及正交试验，确定其最佳酶促合成条件。结果表明，最佳反应条件：叔戊醇为溶剂，Novozym435用量10％（猪油质量分数），反应温度45℃，甘油与猪油物质的量的比4：I，反应时间6h，超声波频率28kHz，超声功率250w。在此条件下，甘油单酯产率达到（84．76±1．96）％。超声辅助酶促反应，不仅可以提高产物产率，还可缩短反应时间。...

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Bibliographic Details
Published in江西农业学报 Vol. 25; no. 12; pp. 101 - 105
Main Author 赵哲 张丹妮 高纯阳 夏元芳 魏微 赵海珍 陆兆新
Format Journal Article
LanguageChinese
Published 南京农业大学 食品科技学院,江苏 南京,210095 2013
Subjects
猪油
甘油单酯
甘油解
脂肪酶
超声波
猪油
Monoglyceride
Glycerolysis
Lard
甘油单酯
甘油解
Ultrasonic treatment
超声波
脂肪酶
Lipase
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Summary:以猪油和丙三醇为原料，采用超声辅助酶促甘油解反应合成甘油单酯，通过单因素试验及正交试验，确定其最佳酶促合成条件。结果表明，最佳反应条件：叔戊醇为溶剂，Novozym435用量10％（猪油质量分数），反应温度45℃，甘油与猪油物质的量的比4：I，反应时间6h，超声波频率28kHz，超声功率250w。在此条件下，甘油单酯产率达到（84．76±1．96）％。超声辅助酶促反应，不仅可以提高产物产率，还可缩短反应时间。
Bibliography:ZHAO Zhe, ZHANG Dan - ni, GAO Chun - yang, XIA Yuan - fang, WEI Wei, ZHAO Hal - zhen , LU Zhao - xin （ College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China）
36-1124/S
Monoglyceride was synthesized by lipase -catalyzed glycerolysis of lard with glycerol through ultrasound assistance. Single - factor experiment and orthogonal test were conducted to optimize the glycerolysis conditions of lard. The optimum conditions for monoglyceride synthesis were acquired as follows： tert - amyl alcohol as reaction medium, Novozym 435 lipase dosage 10% （ calculated on the basis of the mass of lard）, reaction temperature 45 ~C, molar ratio of glycerol to lard 4： 1, reaction time 6 h, ul- trasonic frequency 28 kHz, and ultrasonic power 250 W. Under these optimum conditions, the productive rate of monoglyceride was up to 84.76% -＋ 1.96%. The ultrasound - assisted lipase - catalyzed treatment could not only improve the yield of product, but also shorten the reaction time.
Lipase ; Monoglycer
ISSN:1001-8581