Sequence Analyses on N Gene of Rabies Viruses Isolated from Guangdong Province

• Published in: Animal husbandry and feed science Vol. 2; no. 10; pp. 17 - 18
• Main Authors: Chen, J, Yuan, J, Ke, C-W, Gu, W-X, Huang, Y, Xu, M, Chen, S-Q, Zhang, J-F, Xuan, H, Xiang, H
• Format: Journal Article
• Language: English
• Published: 01.12.2010
• Subjects: Brain; PCR扩增; Rabies virus; RT-PCR; 分离菌株; 基因序列分析; 广东省; 广西地区; 流行毒株; 狂犬病毒
• ISSN: 1943-9911

[ Objective] To find the dominant rabies virus strains that are epidemic in Guangdong Province and thus to better prevent and control rabies. [ Method] Canine brains and salivary glands collected from several regions of Guangdong Province were detected by direct imrnunofluorescence assay and nested RT-PCR. The isolates were further identified by intracranial inoculation in suckling mice. The N gene was amplified by RTPCR and the sequences were aligned with those of prevalent strains in Guangxi region and vaccine strains. [ Result] Two strains were isolated from specimens collected from Guangdong Province. Their N gene sequences had a high similarity of 97.9% -99.4% to those of Guangxi strains and also had a similarity of 87.7% -88.1% to those of vaccine strains of rabies virus. [ Conclusion] Apparently healthy dogs may carry rabies virus, and the prevalence of rabies virus shows obvious regional differences.