利用双酶法制备猴头菇氨基酸营养液
【目的】确定猴头菇氨基酸营养液的最佳制备工艺，为以猴头菇为原料的氨基酸强化食品开发提供参考。【方法】以猴头菇营养液中的氨基酸总量为指标，采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化。【结果】影响猴头菇营养液中氨基酸总量的主次因素依次为：酸眭蛋白酶用量〉纤维素酶用量〉液料IzL〉酶解时间〉酶解温度〉酶解pH，其最佳制备工艺参数为：酸性蛋白酶用量1．00％，纤维素酶用量1．50％，酶解温度50℃，酶解时间60min，酶解pH3．5，液料比25：1。与热水浸提法相比，双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73．2...
Saved in:
| Published in | 南方农业学报 Vol. 43; no. 10; pp. 1553 - 1557 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
贺州学院化学与生物工程系,广西贺州,542800
2012
|
| Subjects | |
| Online Access | Get full text |
| ISSN | 2095-1191 |
| DOI | 10.3969/j.issn.2095-1191.2012.10.1553 |
Cover
| Abstract | 【目的】确定猴头菇氨基酸营养液的最佳制备工艺，为以猴头菇为原料的氨基酸强化食品开发提供参考。【方法】以猴头菇营养液中的氨基酸总量为指标，采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化。【结果】影响猴头菇营养液中氨基酸总量的主次因素依次为：酸眭蛋白酶用量〉纤维素酶用量〉液料IzL〉酶解时间〉酶解温度〉酶解pH，其最佳制备工艺参数为：酸性蛋白酶用量1．00％，纤维素酶用量1．50％，酶解温度50℃，酶解时间60min，酶解pH3．5，液料比25：1。与热水浸提法相比，双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73．2％、可溶性固形物含量提高了75．0％。【结论】以双酶法制备猴头菇氨基酸营养液是可行的，能有效提高营养液中的氨基酸总量和可溶性固形物含量。 |
|---|---|
| AbstractList | 【目的】确定猴头菇氨基酸营养液的最佳制备工艺，为以猴头菇为原料的氨基酸强化食品开发提供参考。【方法】以猴头菇营养液中的氨基酸总量为指标，采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化。【结果】影响猴头菇营养液中氨基酸总量的主次因素依次为：酸眭蛋白酶用量〉纤维素酶用量〉液料IzL〉酶解时间〉酶解温度〉酶解pH，其最佳制备工艺参数为：酸性蛋白酶用量1．00％，纤维素酶用量1．50％，酶解温度50℃，酶解时间60min，酶解pH3．5，液料比25：1。与热水浸提法相比，双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73．2％、可溶性固形物含量提高了75．0％。【结论】以双酶法制备猴头菇氨基酸营养液是可行的，能有效提高营养液中的氨基酸总量和可溶性固形物含量。 TS219; [目的]确定猴头菇氨基酸营养液的最佳制备工艺,为以猴头菇为原料的氨基酸强化食品开发提供参考.[方法]以猴头菇营养液中的氨基酸总量为指标,采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化.[结果]影响猴头菇营养液中氨基酸总量的主次因素依次为:酸性蛋白酶用量＞纤维素酶用量＞液料比＞酶解时间＞酶解温度＞酶解pH,其最佳制备工艺参数为:酸性蛋白酶用量1.00％,纤维素酶用量1.50％,酶解温度50℃,酶解时间60 min,酶解pH 3.5,液料比25∶1.与热水浸提法相比,双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73.2％、可溶性固形物含量提高了75.0％.[结论]以双酶法制备猴头菇氨基酸营养液是可行的,能有效提高营养液中的氨基酸总量和可溶性固形物含量. |
| Author | 赵广河 |
| AuthorAffiliation | 贺州学院化学与生物工程系,广西贺州542800 |
| AuthorAffiliation_xml | – name: 贺州学院化学与生物工程系,广西贺州,542800 |
| Author_FL | ZHAO Guang-he |
| Author_FL_xml | – sequence: 1 fullname: ZHAO Guang-he |
| Author_xml | – sequence: 1 fullname: 赵广河 |
| BookMark | eNo9j71KA0EcxLeIYIx5CAuxunP_-7_duy0l-AUBm_Rh725z5tSN5hCTBwhJEUgKrW0ixE40BNHn2Xy8hScRqxmYHzPMDimYltGE7AN1UQp5mLrNLDMuo5I7ABJyB8zNU-AcC6T4H2yTcpallFLGqS-QFQm1g9fl49SOhuvefPHxZAdzO-kvhzM7ma1G_cXb1D5_rXufq_GL7X0v5u-7ZKuhrjNd_tMSqZ0c1ypnTvXi9LxyVHUiHggHEHyJQRxz7YOvAwmBDsOARVygLzwlEcMG1VTzCGPFAg8VRKHHFA1jRUWEJXKwqX1QpqFMUk9b922TD9aTjuledX4fQv5D5OTehowuWya5a-bsbbt5o9rduocSgKGPP5DvZb4 |
| ClassificationCodes | TS219 |
| ContentType | Journal Article |
| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| Copyright_xml | – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
| DBID | 2RA 92L CQIGP W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| DOI | 10.3969/j.issn.2095-1191.2012.10.1553 |
| DatabaseName | 维普_期刊 中文科技期刊数据库-CALIS站点 维普中文期刊数据库 中文科技期刊数据库-农业科学 中文科技期刊数据库- 镜像站点 Wanfang Data Journals - Hong Kong WANFANG Data Centre Wanfang Data Journals 万方数据期刊 - 香港版 China Online Journals (COJ) China Online Journals (COJ) |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| DocumentTitleAlternate | Double enzymatic preparation method for amino acid nutrition liquid using Hericium erinaceus |
| DocumentTitle_FL | Double enzymatic preparation method for amino acid nutrition liquid using Hericium erinaceus |
| EndPage | 1557 |
| ExternalDocumentID | gxnykx201210026 43911237 |
| GrantInformation_xml | – fundername: 贺州学院科研计划项目 funderid: (2012PYZX09) |
| GroupedDBID | -04 2RA 5XA 5XE 92L ALMA_UNASSIGNED_HOLDINGS CCEZO CHDYS CQIGP U1G U5N W95 ~WA 2B. 4A8 92I 93N PSX TCJ |
| ID | FETCH-LOGICAL-c586-1317938dd5e717e8918ebb82c563764a933bf0e0e5c3da2843a1cb42a0bda06c3 |
| ISSN | 2095-1191 |
| IngestDate | Thu May 29 04:06:47 EDT 2025 Wed Feb 14 10:44:36 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 10 |
| Keywords | 纤维素酶 猴头菇 酸性蛋白酶 营养液 氨基酸 |
| Language | Chinese |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c586-1317938dd5e717e8918ebb82c563764a933bf0e0e5c3da2843a1cb42a0bda06c3 |
| Notes | [Objective]This experiment was conducted to optimize the processing conditions of amino acid nutrition liquid using Herlcium erinaceus in order to provide references for developing amino acid intensified foods by using Herici- nm er/nacens as material. [Method]The total amino acid in amino acid nutrition liquid of Hericium erinaceus was used to determine the dosage of acidic protease and cellulose, enzymolysis temperature, time and pH value, and water-to-Heffci- um ennaeens ratio by using the single factor method and orthogonal tests. [Result]The factor order affecting the total amino acid in liquid were displayed as followed： acidic protease dose 〉cellulose dose water-to-Hencium erinaceus ratio, hydrolysis time, hydrolysis temperature〉hydrolysis pH. The optimum processing conditions of amino acids nutrition liquid were determined as followed： cellulose dose of 1.50%, acidic protease dose of 1.00%, hydrolysis temperature of 50~C, hydrolysis time of 60min, hydrolysis pH of 3.5, and water-to-her~cium e~naceus r |
| PageCount | 5 |
| ParticipantIDs | wanfang_journals_gxnykx201210026 chongqing_primary_43911237 |
| PublicationCentury | 2000 |
| PublicationDate | 2012 |
| PublicationDateYYYYMMDD | 2012-01-01 |
| PublicationDate_xml | – year: 2012 text: 2012 |
| PublicationDecade | 2010 |
| PublicationTitle | 南方农业学报 |
| PublicationTitleAlternate | Journal of Southern Agriculture |
| PublicationTitle_FL | Guangxi Agricultural Sciences |
| PublicationYear | 2012 |
| Publisher | 贺州学院化学与生物工程系,广西贺州,542800 |
| Publisher_xml | – name: 贺州学院化学与生物工程系,广西贺州,542800 |
| SSID | ssj0002507632 ssib007891390 ssib051370557 ssib011608256 ssib018830053 ssib010683288 |
| Score | 1.8653947 |
| Snippet | ... TS219;... |
| SourceID | wanfang chongqing |
| SourceType | Aggregation Database Publisher |
| StartPage | 1553 |
| SubjectTerms | 氨基酸 猴头菇 纤维素酶 营养液 酸性蛋白酶 |
| Title | 利用双酶法制备猴头菇氨基酸营养液 |
| URI | http://lib.cqvip.com/qk/90177A/201210/43911237.html https://d.wanfangdata.com.cn/periodical/gxnykx201210026 |
| Volume | 43 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFB-0gngRRcXWD_bgHLMmmczXMelmKYKetlBPSyabbEFI_Vih9r60h0I96NlLhXoTLYvo35Nt-1_43mw2G6EUFUKYffPem5m87Js3M5nfEPJIpj6M15RwTJIaJxAmd4zJc0eLQKicMS7scsHTZ2JtPXiywTcWkzl2d8nItNOdc_eV_I9VgQZ2xV2y_2DZWikQIA32hTtYGO5_ZWMac6oUDTWNJdUBDZWldKlapbGmitNI0FjQiFHNK2akcBpCCClRCjijoKJgQllxaaXcSqHu0iicK1TIoyFrphAokWWGy29Guja3Q7VVpSFXWwqkoW4B6tGhLbdDQ1tJFYLOuf2xkIjjBSwgGnVtIT40pTlP4TUmLX0I4xwEkmt63Rk40_ztchs-FE8yavTH8FOe5-uZFtr6eiyjXZeBX-v5bXuw01zNH3DauNMYemt5mVzxpfTwK9DO8_XaBUlctl0sHMKAGVzeAtHN8wSOqesIzlMK8f5rF8c9hvBEsp7mgwgTPDiuZdU1vEpoVf3HF1Ye0T42t4rhK4hh7JayIk-KYSP66d0g16thSyucvYM3yaWdzVvELfe-nHw4Kg_2z8aT6feP5d6kPNw92T8uD49PD3anX4_KTz_Pxj9O338ux7-mk2-3Sa8b91bXnOoEDiflCvEp0X2rwYBnMOrP4NGozBjlp1xAvxQkmjGTu5mb8ZQNEgh0WOKlJvAT1wwSV6TsDlkqtorsLmkJro3HMwjG8yTQwiBsIhvwJJWp9PzUXyYrdWP7L2dAK_25rZZJq2p9v_r3vekPt4t3L7bxeSGIsFi5SP4euYaMs5mz-2Rp9Ppt9gBiyZF5aK3_G1r8V8w |
| linkProvider | CAB International |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%88%A9%E7%94%A8%E5%8F%8C%E9%85%B6%E6%B3%95%E5%88%B6%E5%A4%87%E7%8C%B4%E5%A4%B4%E8%8F%87%E6%B0%A8%E5%9F%BA%E9%85%B8%E8%90%A5%E5%85%BB%E6%B6%B2&rft.jtitle=%E5%8D%97%E6%96%B9%E5%86%9C%E4%B8%9A%E5%AD%A6%E6%8A%A5&rft.au=%E8%B5%B5%E5%B9%BF%E6%B2%B3&rft.date=2012&rft.issn=2095-1191&rft.volume=43&rft.issue=10&rft.spage=1553&rft.epage=1557&rft_id=info:doi/10.3969%2Fj.issn.2095-1191.2012.10.1553&rft.externalDocID=43911237 |
| thumbnail_s | http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fimage.cqvip.com%2Fvip1000%2Fqk%2F90177A%2F90177A.jpg http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fgxnykx%2Fgxnykx.jpg |