利用双酶法制备猴头菇氨基酸营养液

【目的】确定猴头菇氨基酸营养液的最佳制备工艺，为以猴头菇为原料的氨基酸强化食品开发提供参考。【方法】以猴头菇营养液中的氨基酸总量为指标，采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化。【结果】影响猴头菇营养液中氨基酸总量的主次因素依次为：酸眭蛋白酶用量〉纤维素酶用量〉液料IzL〉酶解时间〉酶解温度〉酶解pH，其最佳制备工艺参数为：酸性蛋白酶用量1．00％，纤维素酶用量1．50％，酶解温度50℃，酶解时间60min，酶解pH3．5，液料比25：1。与热水浸提法相比，双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73．2...

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Bibliographic Details
Published in南方农业学报 Vol. 43; no. 10; pp. 1553 - 1557
Main Author 赵广河
Format Journal Article
LanguageChinese
Published 贺州学院化学与生物工程系,广西贺州,542800 2012
Subjects
氨基酸
猴头菇
纤维素酶
营养液
酸性蛋白酶
纤维素酶
猴头菇
酸性蛋白酶
营养液
氨基酸
Online AccessGet full text
ISSN2095-1191
DOI10.3969/j.issn.2095-1191.2012.10.1553

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Summary:【目的】确定猴头菇氨基酸营养液的最佳制备工艺，为以猴头菇为原料的氨基酸强化食品开发提供参考。【方法】以猴头菇营养液中的氨基酸总量为指标，采用单因素试验和正交试验对影响氨基酸总量的酸性蛋白酶用量、纤维素酶用量、酶解温度、酶解时间、酶解pH、液料比等因素进行优化。【结果】影响猴头菇营养液中氨基酸总量的主次因素依次为：酸眭蛋白酶用量〉纤维素酶用量〉液料IzL〉酶解时间〉酶解温度〉酶解pH，其最佳制备工艺参数为：酸性蛋白酶用量1．00％，纤维素酶用量1．50％，酶解温度50℃，酶解时间60min，酶解pH3．5，液料比25：1。与热水浸提法相比，双酶法制备的猴头菇氨基酸营养液中氨基酸总量提高了73．2％、可溶性固形物含量提高了75．0％。【结论】以双酶法制备猴头菇氨基酸营养液是可行的，能有效提高营养液中的氨基酸总量和可溶性固形物含量。
Bibliography:[Objective]This experiment was conducted to optimize the processing conditions of amino acid nutrition liquid using Herlcium erinaceus in order to provide references for developing amino acid intensified foods by using Herici- nm er/nacens as material. [Method]The total amino acid in amino acid nutrition liquid of Hericium erinaceus was used to determine the dosage of acidic protease and cellulose, enzymolysis temperature, time and pH value, and water-to-Heffci- um ennaeens ratio by using the single factor method and orthogonal tests. [Result]The factor order affecting the total amino acid in liquid were displayed as followed： acidic protease dose 〉cellulose dose water-to-Hencium erinaceus ratio, hydrolysis time, hydrolysis temperature〉hydrolysis pH. The optimum processing conditions of amino acids nutrition liquid were determined as followed： cellulose dose of 1.50%, acidic protease dose of 1.00%, hydrolysis temperature of 50~C, hydrolysis time of 60min, hydrolysis pH of 3.5, and water-to-her~cium e~naceus r
ISSN:2095-1191
DOI:10.3969/j.issn.2095-1191.2012.10.1553