Combined Transcriptomic and Protein Array Cytokine Profiling of Human Stem Cells from Dental Apical Papilla Modulated by Oral Bacteria

• Published in: International journal of molecular sciences Vol. 23; no. 9; p. 5098
• Main Authors: Zymovets, Valeriia, Razghonova, Yelyzaveta, Rakhimova, Olena, Aripaka, Karthik, Manoharan, Lokeshwaran, Kelk, Peyman, Landström, Maréne, Romani Vestman, Nelly
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.05.2022; MDPI
• Subjects: Adult; Bacteria; Bacteria - metabolism; Bacterial infections; Basic Medicine; beta Catenin - genetics; beta Catenin - metabolism; Biofilms; Biomedical materials; Cell and Molecular Biology; Cell differentiation; Cell Differentiation - physiology; Cell- och molekylärbiologi; Chemokines; cytokine secretion; Cytokines; Dental Papilla; Differentiation (biology); Endodontics; Fusobacterium nucleatum; Humans; IL-6; IL-8; immune response; Immunoassay; Immunogenicity; Inflammation; Interleukin 6; Interleukin 8; Medical and Health Sciences; Medicin och hälsovetenskap; Medicinska och farmaceutiska grundvetenskaper; Mesenchyme; Microbiota; Monocyte chemoattractant protein 1; NF-kappa B - metabolism; NF-κB protein; Nosocomial infections; Osteogenesis - physiology; osteogenic potential; Pathogens; Protein Array Analysis; Protein arrays; Proteins; regenerative endodontic treatment (RET); RNA, Messenger; Signal transduction; Stem cells; Stem Cells - metabolism; stem cells from the apical papilla (SCAP); TRAF6 protein; Transcriptome; transcriptome analysis; Transcriptomics; Tumor necrosis factor-TNF; Vascular endothelial growth factor; Wnt protein; β-Catenin; endodontics; Fusobacterium nucleatum; cytokine secretion; regenerative endodontic treatment (RET); immune response; osteogenic potential; stem cells from the apical papilla (SCAP); transcriptome analysis; IL-8; IL-6
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms23095098

Stem cells from the apical papilla (SCAP) are a promising resource for use in regenerative endodontic treatment (RET) that may be adversely affected by oral bacteria, which in turn can exert an effect on the success of RET. Our work aims to study the cytokine profile of SCAP upon exposure to oral bacteria and their supernatants- and -as well as to establish their effect on the osteogenic and immunogenic potentials of SCAP. Further, we target the presence of key proteins of the Wnt/β-Catenin, TGF-β, and NF-κB signaling pathways, which play a crucial role in adult osteogenic differentiation of mesenchymal stem cells, using the Western blot (WB) technique. The membrane-based sandwich immunoassay and transcriptomic analysis showed that, under the influence of (both bacteria and supernatant), the production of pro-inflammatory cytokines IL-6, IL-8, and MCP-1 occurred, which was also confirmed at the mRNA level. Conversely, reduced the secretion of the aforementioned cytokines at both mRNA and protein levels. WB analysis showed that SCAP co-cultivation with led to a decrease in the level of the key proteins of the Wnt/β-Catenin and NF-κB signaling pathways: β-Catenin ( = 0.0068 *), LRP-5 ( = 0.0059 **), and LRP-6 ( = 0.0329 *), as well as NF-kB ( = 0.0034 **) and TRAF6 ( = 0.0285 *). These results suggest that oral bacteria can up- and downregulate the immune and inflammatory responses of SCAP, as well as influence the osteogenic potential of SCAP, which may negatively regulate the success of RET.