Subpopulations in aMPV vaccines are unlikely to be the only cause of reversion to virulence

• Published in: Vaccine Vol. 33; no. 21; pp. 2438 - 2441
• Main Authors: Franzo, G, Naylor, C.J, Drigo, M, Croville, G, Ducatez, M.F, Catelli, E, Laconi, A, Cecchinato, M
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 15.05.2015; Elsevier Limited; Elsevier
• Subjects: Allergy and Immunology; Animal biology; Animals; Avian metapneumovirus; Data analysis; Deoxyribonucleic acid; DNA; Genetic Variation; Genomes; Haplotypes; High-Throughput Nucleotide Sequencing; Life Sciences; Metapneumovirus - classification; Metapneumovirus - genetics; Metapneumovirus - isolation & purification; Metapneumovirus - physiology; Methods; Microbiology and Parasitology; Mutation; NGS; Protocol; Reversion to virulence; RNA, Viral - genetics; Santé publique et épidémiologie; Software; Studies; Subpopulations; Vaccine; Vaccines; Vaccines, Attenuated - administration & dosage; Vaccines, Attenuated - adverse effects; Vaccines, Attenuated - genetics; Veterinary medicine and animal Health; Viral subpopulation; Viral Vaccines - administration & dosage; Viral Vaccines - adverse effects; Viral Vaccines - genetics; Virology; Virulence; Germany; Reversion to virulence; Vaccine; NGS; Viral subpopulation; Avian metapneumovirus
• ISSN: 0264-410X; 1873-2518; 0264-410X
• DOI: 10.1016/j.vaccine.2015.03.092

Highlights • Development of different NGS protocols for aMPV full genome sequencing. • First study investigating aMPV vaccine subpopulation using NGS. • Comparison of identified SNV with attenuation/reversion to virulence markers. • Evidence of the role of intra-vaccine variability in vaccine reversion to virulence.