Effect of 6-day intense Kendo training on lymphocyte counts and its expression of CD95

• Published in: European journal of applied physiology Vol. 107; no. 2; pp. 227 - 233
• Main Authors: Tanimura, Yuko, Kon, Michihiro, Shimizu, Kazuhiro, Kimura, Fuminori, Kono, Ichiro, Ajisaka, Ryuichi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.09.2009; Springer; Springer Nature B.V
• Subjects: Adolescent; Athletes; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; fas Receptor - metabolism; Fundamental and applied biological sciences. Psychology; Human Physiology; Humans; Hydrocortisone - blood; Lymphocyte Count; Martial Arts - physiology; Occupational Medicine/Industrial Medicine; Original Article; Physical Education and Training - methods; Physical Exertion - physiology; Sports Medicine; T-Lymphocytes - cytology; T-Lymphocytes - metabolism; Time Factors; Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports; Young Adult; Intensified training; CD95; Apoptosis; Lymphocytopenia; Human; Physical training; Leukopenia; Fas Antigen; Hemopathy; Lymphocyte
• ISSN: 1439-6319; 1439-6327
• DOI: 10.1007/s00421-009-1119-1

This study examines the effects of 6-day intensive training on lymphocyte counts and their expression of CD95. Eight healthy Kendo athletes underwent 6-day Kendo training of about 310 min each day. Blood samples were collected at 2 weeks before (PRE), the first day (Day 1), third day (Day 3), fifth day (Day 5), and 1 week after the training period (POST) to determine lymphocyte counts and CD95 expression on CD95 lymphocytes (CD4 + , CD8 + ) using flow cytometry. The total lymphocyte counts were significantly lower at Day 3 than at PRE. The CD8 + cell counts were significantly lower at Day 3 than at PRE. The percentage of CD95 + lymphocytes was significantly higher at Day 1 and Day 3 than at PRE. The percentage of CD8 + CD95 + cells did not change significantly. The total lymphocyte counts decreased and a concomitant increase of CD95 + lymphocyte was observed, whereas the decrease in CD8 + cell counts was not associated with the increase in CD8 + CD95 + cells. Therefore, short-term high-intensity exercise induced a decrease in the T lymphocyte counts without increasing in CD95 + expression.