Exosomal miR-23b from bone marrow mesenchymal stem cells alleviates oxidative stress and pyroptosis after intracerebral hemorrhage

• Published in: Neural regeneration research Vol. 18; no. 3; pp. 560 - 567
• Main Authors: Hu, Liu-Ting, Wang, Bing-Yang, Fan, Yu-Hua, He, Zhi-Yi, Zheng, Wen-Xu
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.03.2023; Medknow Publications & Media Pvt. Ltd; Department of Neurology,The First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong Province,China; Department of Neurology,The First Affiliated Hospital of China Medical University,Shenyang,Liaoning Province,China%Department of Neurology,The First Affiliated Hospital of China Medical University,Shenyang,Liaoning Province,China%Department of Neurology,The First Affiliated Hospital,Sun Yat-Sen University,Guangzhou,Guangdong Province,China%Geriatric Department of Dalian Friendship Hospital,Dalian,Liaoning Province,China; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Bone marrow; bone marrow mesenchymal stem cells; exosomal mirnas; intracerebral hemorrhage; mir-23b; neuroinflammation; nlrp3 inflammasome; nrf2; oxidative stress; pten; pyroptosis; Oxidative stress; intracerebral hemorrhage; pyroptosis; Nrf2; neuroinflammation; miR-23b; PTEN; NLRP3 inflammasome; bone marrow mesenchymal stem cells; oxidative stress; exosomal miRNAs
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.346551

Our previous studies showed that miR-23b was downregulated in patients with intracerebral hemorrhage (ICH). This indicates that miR-23b may be closely related to the patho-physiological mechanism of ICH, but this hypothesis lacks direct evidence. In this study, we established rat models of ICH by injecting collagenase VII into the right basal ganglia and treating them with an injection of bone marrow mesenchymal stem cell (BMSC)-derived exosomal miR-23b via the tail vein. We found that edema in the rat brain was markedly reduced and rat behaviors were improved after BMSC exosomal miR-23b injection compared with those in the ICH groups. Additionally, exosomal miR-23b was transported to the microglia/macrophages, thereby reducing oxidative stress and pyroptosis after ICH. We also used hemin to mimic ICH conditions in vitro. We found that phosphatase and tensin homolog deleted on chromosome 10 (PTEN) was the downstream target gene of miR-23b, and exosomal miR-23b exhibited antioxidant effects by regulating the PTEN/Nrf2 pathway. Moreover, miR-23b reduced PTEN binding to NOD-like receptor family pyrin domain containing 3 (NLRP3) and NLRP3 inflammasome activation, thereby decreasing the NLRP3-dependent pyroptosis level. These findings suggest that BMSC-derived exosomal miR-23b exhibits antioxidant effects through inhibiting PTEN and alleviating NLRP3 inflammasome-mediated pyroptosis, thereby promoting neurologic function recovery in rats with ICH.