Integration Between Different Hypothalamic Nuclei Involved in Stress and GnRH Secretion in the Ewe

• Published in: Reproduction in domestic animals Vol. 45; no. 6; pp. 1065 - 1073
• Main Authors: Ghuman, SPS, Morris, R, Spiller, DG, Smith, RF, Dobson, H
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2010; Blackwell
• Subjects: Anatomy; Animal reproduction; Animals; Arcuate nucleus; Arginine Vasopressin; Argipressin; beta -Endorphin; Biological and medical sciences; Brain stem; Cattle; Cell body; Confocal microscopy; Corticotropin-Releasing Hormone; Female; Fundamental and applied biological sciences. Psychology; gamma -Aminobutyric acid; Gonadotropin-releasing hormone; Gonadotropin-Releasing Hormone - metabolism; Hormones; Hypothalamus; Hypothalamus - anatomy & histology; Hypothalamus - physiology; Immunofluorescence; Integration; Interneurons; Mammalian reproduction. General aspects; Norepinephrine; Paraventricular nucleus; Pituitary (anterior); Receptors, Adrenergic; Receptors, GABA; Seasons; Sheep - physiology; Stress; Stress analysis; Stress, Physiological - physiology; Vertebrates: reproduction; Secretion; Central nervous system; Gonadotropin RH; Hypothalamus; Stress; Encephalon; Hypothalamic hormone; Vertebrata; Ewe; Mammalia; Animal; Sheep; Artiodactyla; Hormone releasing factor; Ungulata
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2009.01496.x

This study investigated possible integrated links in the neuroanatomical pathways through which the activity of neurones in the paraventricular nucleus and arcuate nucleus may modulate suppression of gonadotrophin‐releasing hormone (GnRH) secretion during stressful situations. Double‐label immunofluorescence and laser scanning confocal microscopy were used to examine the hypothalamic sections from the follicular phase ewes. Noradrenergic terminals were in close contact with 65.7 ± 6.1% corticotrophin‐releasing hormone (CRH) and 84.6 ± 3.2% arginine vasopressin (AVP) cell bodies in the paraventricular nucleus but not with β‐endorphin cell bodies in the arcuate nucleus. Furthermore, γ‐amino butyric acid (GABA) terminals were close to 80.9 ± 3.5% CRH but no AVP cell bodies in the paraventricular nucleus, as well as 60.8 ± 4.1%β‐endorphin cell bodies in the arcuate nucleus. Although CRH, AVP and β‐endorphin cell terminals were identified in the medial pre‐optic area, no direct contacts with GnRH cell bodies were observed. Within the median eminence, abundant CRH but not AVP terminals were close to GnRH cell terminals in the external zone; whereas, β‐endorphin cells and terminals were in the internal zone. In conclusion, neuroanatomical evidence is provided for the ewe supporting the hypothesis that brainstem noradrenergic and hypothalamic GABA neurones are important in modulating the activity of CRH and AVP neurones in the paraventricular nucleus, as well as β‐endorphin neurones in the arcuate nucleus. These paraventricular and arcuate neurones may also involve interneurones to influence GnRH cell bodies in medial pre‐optic area, whereas the median eminence may provide a major site for direct modulation of GnRH release by CRH terminals.