Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle

• Published in: The Journal of physiology Vol. 576; no. 2; pp. 613 - 624
• Main Authors: Dreyer, Hans C., Fujita, Satoshi, Cadenas, Jerson G., Chinkes, David L., Volpi, Elena, Rasmussen, Blake B.
• Format: Journal Article
• Language: English
• Published: Oxford, UK The Physiological Society 15.10.2006; Blackwell Publishing Ltd; Blackwell Science Inc
• Subjects: Adaptor Proteins, Signal Transducing - metabolism; Adult; Amino Acids - metabolism; AMP-Activated Protein Kinases; Biopsy; Exercise - physiology; Female; Glucose - pharmacokinetics; Humans; Hydrogen-Ion Concentration; Lactates - metabolism; Male; Multienzyme Complexes - genetics; Multienzyme Complexes - metabolism; Muscle Proteins - metabolism; Muscle, Skeletal - blood supply; Muscle, Skeletal - metabolism; Muscle, Skeletal - pathology; Phosphoproteins - metabolism; Phosphorylation; Protein Kinases - metabolism; Protein-Serine-Threonine Kinases - genetics; Protein-Serine-Threonine Kinases - metabolism; Regional Blood Flow - physiology; Skeletal Muscle and Exercise; TOR Serine-Threonine Kinases; Weight Lifting - physiology
• ISSN: 0022-3751; 1469-7793
• DOI: 10.1113/jphysiol.2006.113175

Resistance exercise is a potent stimulator of muscle protein synthesis and muscle cell growth, with the increase in protein synthesis being detected within 2–3 h post-exercise and remaining elevated for up to 48 h. However, during exercise, muscle protein synthesis is inhibited. An increase in AMP-activated protein kinase (AMPK) activity has recently been shown to decrease mammalian target of rapamycin (mTOR) signalling to key regulators of translation initiation. We hypothesized that the cellular mechanism for the inhibition of muscle protein synthesis during an acute bout of resistance exercise in humans would be associated with an activation of AMPK and an inhibition of downstream components of the mTOR pathway (4E-BP1 and S6K1). We studied 11 subjects (seven men, four women) before, during, and for 2 h following a bout of resistance exercise. Muscle biopsy specimens were collected at each time point from the vastus lateralis. We utilized immunoprecipitation and immunoblotting methods to measure muscle AMPKα2 activity, and mTOR-associated upstream and downstream signalling proteins, and stable isotope techniques to measure muscle fractional protein synthetic rate (FSR). AMPKα2 activity (pmol min −1 (mg protein) −1 ) at baseline was 1.7 ± 0.3, increased immediately post-exercise (3.0 ± 0.6), and remained elevated at 1 h post-exercise ( P < 0.05). Muscle FSR decreased during exercise and was significantly increased at 1 and 2 h post-exercise ( P < 0.05). Phosphorylation of 4E-BP1 at Thr37/46 was significantly reduced immediately post-exercise ( P < 0.05). We conclude that AMPK activation and a reduced phosphorylation of 4E-BP1 may contribute to the inhibition of muscle protein synthesis during resistance exercise. However, by 1–2 h post-exercise, muscle protein synthesis increased in association with an activation of protein kinase B, mTOR, S6K1 and eEF2.