云南越桔花粉活力测定与花粉保存

用不同浓度蔗糖、硼酸对云南越桔花粉进行离体培养后统计其萌发率,结果表明：云南越桔花粉最适宜的离体培养基为15%蔗糖＋0.1%硼酸＋1%琼脂,花粉萌发最好,萌发率高达78.26%。此外,4℃恒温、避光条件下贮藏了不同时间的花粉,在适宜培养基中的离体培养萌发率试验表明：云南越桔花粉现采的活力最高,花粉的贮藏时间以不超过1个月为宜。...

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Bibliographic Details
Published in江西农业学报 Vol. 26; no. 6; pp. 56 - 58
Main Author 杨洪涛 李燕 杨燕林 和志娇 王朝文 黄杏娥 和加卫
Format Journal Article
LanguageChinese
Published 云南省农业科学院 高山经济植物研究所,云南 丽江,674100 2014
Subjects
云南越桔
花粉
花粉活力
贮藏时间
云南越桔
花粉活力
Storage time
花粉
Vaccinium duclouxii
Pollen
Vitality
贮藏时间
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Summary:用不同浓度蔗糖、硼酸对云南越桔花粉进行离体培养后统计其萌发率,结果表明：云南越桔花粉最适宜的离体培养基为15%蔗糖＋0.1%硼酸＋1%琼脂,花粉萌发最好,萌发率高达78.26%。此外,4℃恒温、避光条件下贮藏了不同时间的花粉,在适宜培养基中的离体培养萌发率试验表明：云南越桔花粉现采的活力最高,花粉的贮藏时间以不超过1个月为宜。
Bibliography:36-1124/S
Vaccinium duclouxii;Pollen;Vitality;Storage time
Different concentrations of sucrose and boric acid were used for the isolated culture of pollens in Vaccinium du-clouxii, and the germination rate of the pollens in vitro was statistically analyzed .The results indicated that the optimum culture me-dium for Vaccinium duclouxii pollens in vitro was 15%sucrose ＋0.1%boric acid ＋1%agar, and the germination rate of the pol-lens in this culture medium was the highest , reaching 78.26%.In addition, the isolated Vaccinium duclouxii pollens which have been preserved under the conditions of constant temperature 4℃and darkness for a different time were cultivated on suitable culture medium , and their germination rate was tested .The experimental results showed that the fresh -collected pollens had the highest vi-tality, and the storage time for the pollens should not be longer than one month .
YANG Hong - tao, LI Yan, YANG Yan- lin, HE Zhi - jiao, WANG Chao - wen, HUANG Xing - e, HE Jia - wei （ Institute of Alpine
ISSN:1001-8581