36份菠萝种质的遗传多样性SCoT分析

利用SCoT标记研究了36份菠萝种质资源的遗传多样性。从86条引物中筛选出18条重复性好,条带清晰的引物进行PCR扩增,共扩增出209条带,其中135条具有多态性,多态性带比率为64.99%。经NTSYS软件分析,36份菠萝种质间遗传相似系数在0.63～0.93之间,说明SCoT标记能够揭示菠萝种质间较高的遗传多样性。UPGMA聚类分析显示,SCoT标记能将36份菠萝种质完全区分开,并分成五类。...

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Published in西南农业学报 Vol. 25; no. 2; pp. 625 - 629
Main Author 陈香玲 苏伟强 刘业强 任惠 陆玉英
Format Journal Article
LanguageChinese
Published 广西作物遗传改良生物技术重点开放实验室,广西南宁530007%广西农业科学院园艺研究所,广西南宁,530007 2012
广西农业科学院园艺研究所,广西南宁530007
Subjects
SCoT
菠萝
遗传多样性
遗传多样性
菠萝
SCoT
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Summary:利用SCoT标记研究了36份菠萝种质资源的遗传多样性。从86条引物中筛选出18条重复性好,条带清晰的引物进行PCR扩增,共扩增出209条带,其中135条具有多态性,多态性带比率为64.99%。经NTSYS软件分析,36份菠萝种质间遗传相似系数在0.63～0.93之间,说明SCoT标记能够揭示菠萝种质间较高的遗传多样性。UPGMA聚类分析显示,SCoT标记能将36份菠萝种质完全区分开,并分成五类。
Bibliography:51-1213/S
SCoT markers were applied to study the genetic diversity and relationship of 36 pineapple collections.Eighteen primers were screened from 86 SCoT primers and then used to amplify the genome DNAs of the collections.Totally 209 SCoT loci were produced,including 135 polymorphic loci with a polymorphism rate of 64.99 %.The genetic similarity coefficients among the 36 accessions ranged from 0.63 to 0.93,which showed that SCoT loci could reveal the relatively high genetic diversity among the pineapple collections.UPGMA cluster analysis displayed that through SCoT markers 36 collections could be distinguished from each other and divided into five groups.
Pineapple; SCoT; Genetic diversity
CHEN Xiang-ling,SU Wei-qiang,LIU Ye-qiang,REN Hui,LU Yu-ying （1.Horticultural Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China;2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 530007,China）
ISSN:1001-4829
DOI:10.3969/j.issn.1001-4829.2012.02.055