3,3′-二吲哚甲烷对小鼠造血系统辐射损伤的保护作用

目的探讨3,3′-二吲哚甲烷（3,3′-diindolylmethane,DIM）对2Gy辐射损伤小鼠造血系统的保护作用。方法30只雄性C57BL/6小鼠随机区组法分为对照组、2Gy照射组和2Gy照射+DIM组，每组10只。对照组小鼠接受假照射（0Gy），其余2组小鼠接受2Gy137Csγ-射线一次性全身照射。2Gy照射+DIM组小鼠照射前30min腹腔注射DIM（75mg/kg），其余2组小鼠腹腔注射对照溶液。照后7d和15d每组取5只小鼠，取外周血进行血象测定，取骨髓细胞测定骨髓有核细胞（BMNCs）数目，活性氧检测探针（DCFH-DA）检测细胞内活性氧（ROS）水平、骨髓粒-单核巨噬细胞...

Full description

Saved in:
Bibliographic Details
Published in天津医药 Vol. 45; no. 6; pp. 580 - 583
Main Author 董佳丽;路璐;樊赛军
Format Journal Article
LanguageChinese
Published 中国医学科学院北京协和医学院放射医学研究所,天津市放射医学与分子核医学重点实验室,300192 2017
Subjects
辐射损伤;造血系统;活性氧;骨髓细胞;小鼠,近交C57BL;3,3′-二吲哚甲烷
骨髓细胞
inbred C57BL
bone marrow cells
hematopoietic system
mice
辐射损伤
3′-diindolylmethane
3
造血系统
reactive oxygen species
小鼠,近交C57BL
radiation injuries
活性氧
3,3′-二吲哚甲烷
Online AccessGet full text

Cover

More Information
Summary:目的探讨3,3′-二吲哚甲烷（3,3′-diindolylmethane,DIM）对2Gy辐射损伤小鼠造血系统的保护作用。方法30只雄性C57BL/6小鼠随机区组法分为对照组、2Gy照射组和2Gy照射+DIM组，每组10只。对照组小鼠接受假照射（0Gy），其余2组小鼠接受2Gy137Csγ-射线一次性全身照射。2Gy照射+DIM组小鼠照射前30min腹腔注射DIM（75mg/kg），其余2组小鼠腹腔注射对照溶液。照后7d和15d每组取5只小鼠，取外周血进行血象测定，取骨髓细胞测定骨髓有核细胞（BMNCs）数目，活性氧检测探针（DCFH-DA）检测细胞内活性氧（ROS）水平、骨髓粒-单核巨噬细胞集落（CFU-GM）形成数目检测克隆形成能力。结果与对照组比较，2Gy照射组外周血白细胞、血小板数量、BMNCs数目和克隆形成能力显著下降，细胞内ROS水平显著提高（P<0.05）。与2Gy照射组比较，2Gy照射+DIM组外周血白细胞、血小板数量、BMNCs数目和克隆形成能力显著提高，细胞内ROS水平显著下降（P<0.05）。结论DIM对小鼠2Gy照射后造血系统损伤有一定的保护作用，其机制可能与降低骨髓细胞内ROS水平有关。
Bibliography:radiation injuries; hematopoietic system; reactive oxygen species; bone marrow cells; mice, inbred C57BL;3,3′-diindolylmethane
Objective To investigate the protective effect of3,3′-diindolylmethane(DIM)on radiation-induced injuryin mouse hematopoietic system.Methods Thirty C57BL/6mice were randomly divided into control group,2Gy irradiationgroup and2Gy irradiation+DIM group(n=10for each group).Mice of control group received sham irradiation,and the othertwo groups accepted2Gy137Csγ-ray total body irradiation.Mice in2Gy irradiation+DIM group were intraperitoneallyinjected75mg/kg DIM30min before irradiation.Mice of other two groups were treated with reference solution.After7d and15d of2Gy irradiation,the peripheral blood samples were collected to count the number of bone marrow nuclear cells(BMNCs).The level of reactive oxygen species(ROS)was measured by DCFH-DA.The levels of colony forming unitsgranulocyte-macrophage(CFU-GM)were also detected.Results The numbers of white blood cell(WBC),platelet count(PLT),BMNC
ISSN:0253-9896
DOI:10.11958/20170457