A serosurvey of borna disease virus infection in wild rats [Rattus norvegicus] by a capture ELISA

• Published in: Journal of Veterinary Medical Science Vol. 61; no. 2; pp. 113 - 117
• Main Authors: Tsujimura, K. (Hokkaido Univ., Sapporo (Japan)), Mizutani, T, Kariwa, H, Yoshimatsu, K, Ogino, M, Morii, Y, Inagaki, H, Arikawa, J, Takashima, I
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.02.1999; Japan Science and Technology Agency
• Subjects: ANIMAL SALVAJE; ANIMAL SAUVAGE; Animals; Antibodies, Monoclonal; Antibodies, Viral - blood; Blotting, Western - veterinary; Borna Disease - epidemiology; BORNA DISEASE VIRUS; Borna disease virus - immunology; capture ELISA; Cell Line; DISEASE SURVEYS; Dogs; ELISA; ENCUESTAS SANITARIAS; ENQUETE PATHOLOGIQUE; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - veterinary; INFECCION; INFECTION; Japan - epidemiology; Mice; Mice, Inbred BALB C; RAT; RATA; RATS; Rattus norvegicus; Rodent Diseases - epidemiology; TEST ELISA; Viral Proteins - immunology; VIRUS ENFERMEDAD DE BORNA; VIRUS MALADIE DE BORNA; WILD ANIMALS; Japan
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.61.113

For a serological diagnostic test for Borna disease (BD), we developed a capture ELISA with specificity and sensitivity based on detection of antibodies against BD virus (BDV) p40 protein. Using our capture ELISA system, the antibody response of rats inoculated intracerebrally with BDV at 4 weeks after birth showed a sharp increase from 1 to 4 weeks postinoculation (p.i.) and a steady level after 5 weeks p.i. To investigate prevalence of BDV infection among wild rats, we examined sera of Rattus norvegicus in Kami-iso town, Oshima district, Hokkaido, suggesting that rats in this area had not been infected by BDV