Comparison of diffusion-weighted MRI and anti-Stokes Raman scattering (CARS) measurements of the inter-compartmental exchange-time of water in expression-controlled aquaporin-4 cells

• Published in: Scientific reports Vol. 8; no. 1; pp. 17954 - 11
• Main Authors: Obata, Takayuki, Kershaw, Jeff, Tachibana, Yasuhiko, Miyauchi, Takayuki, Abe, Yoichiro, Shibata, Sayaka, Kawaguchi, Hiroshi, Ikoma, Yoko, Takuwa, Hiroyuki, Aoki, Ichio, Yasui, Masato
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.12.2018; Nature Publishing Group
• Subjects: 59/57; 631/80; 692/53; Aquaporin 4; Aquaporin 4 - genetics; Aquaporin 4 - metabolism; Aquaporins; Diffusion coefficient; Diffusion Magnetic Resonance Imaging - methods; Extracellular Space - metabolism; Humanities and Social Sciences; Intracellular Space - metabolism; Magnetic resonance imaging; Models, Theoretical; Molecular Imaging - methods; multidisciplinary; NMR; Nuclear magnetic resonance; Science; Science (multidisciplinary); Spectrum Analysis, Raman - methods; Water - metabolism
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-36264-9

We performed multi-b and multi-diffusion-time diffusion-weighted magnetic resonance imaging on aquaporin-4-expressing (AQ) and -non-expressing (noAQ) cells, and demonstrated a clear difference between the signals from the two cell types. The data were interpreted using a two-compartment (intra and extracellular spaces) model including inter-compartmental exchange. It was also assumed that restricted diffusion of water molecules inside the cells leads to the intracellular diffusion coefficient being inversely proportional to the diffusion-time. Estimates of the water-exchange-times obtained with this model are compared to those measured using an independent optical imaging technique (coherent anti-Stokes Raman scattering imaging, CARS). For both techniques it was found that the exchange-time estimated for the noAQ cells was significantly longer than that for the AQ cells.