Three genes of the Arabidopsis RPP1 complex resistance locus recognize distinct Peronospora parasitica avirulence determinants

• Published in: The Plant cell Vol. 10; no. 11; pp. 1847 - 1860
• Main Authors: Botella, M.A. (Universidad de Malaga, Malaga, Spain.), Parker, J.E, Frost, L.N, Bittner-Eddy, P.D, Beynon, J.L, Daniels, M.J, Holub, E.B, Jones, J.D.G
• Format: Journal Article
• Language: English
• Published: United States American Society of Plant Physiologists 01.11.1998; American Society of Plant Biologists
• Subjects: ADN; Airborne microorganisms; Alleles; Amino Acid Sequence; AMINO ACID SEQUENCES; Amino acids; Arabidopsis; Arabidopsis - genetics; Arabidopsis - microbiology; ARABIDOPSIS THALIANA; CARTE GENETIQUE; CHEMICAL COMPOSITION; Chromosome Mapping; COMPLEMENTARY DNA; COMPLEX LOCI; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DISEASE RESISTANCE; DNA; DNA, Plant; DNA, Plant - genetics; Evolution, Molecular; EXONS; EXPERIMENTACION IN VIVO; EXPERIMENTAL INFECTION; EXPERIMENTATION IN VIVO; GENBANK/AF098963; GENBANK/AF098964; GENE; GENES; Genes, Plant; Genetic loci; GENETIC MAPPING; GENETIC MAPS; Genetic Variation; GENETICA; GENETICS; GENETIQUE; IN VIVO EXPERIMENTATION; INFECCION EXPERIMENTAL; INFECTION EXPERIMENTALE; INTRONS; MAPAS GENETICOS; microbiology; MOLECULAR MAPPING; MOLECULAR SEQUENCE DATA; Multigene Family; MUTANT; MUTANTES; MUTANTS; Mutation; NUCLEOTIDE SEQUENCE; Oomycetes; Oomycetes - pathogenicity; Parasitism; PATHOGENICITY; Pathogens; PATOGENICIDAD; PERONOSPORA PARASITICA; Phenotype; Phenotypes; Plant cells; Plant Diseases; Plant Diseases - genetics; Plant Diseases - microbiology; PLANT PROTEINS; Plant resistance; Plant species; Plants; POUVOIR PATHOGENE; PROTEINAS; PROTEINE; PROTEINS; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; RPP1 GENES; SECUENCIA NUCLEOTIDICA; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Species diversity; Virulence; Virulence - genetics
• ISSN: 1040-4651; 1532-298X
• DOI: 10.1105/tpc.10.11.1847

Plant resistance (R) genes have evolved specific recognition capabilities in defense against pathogens. The evolution of R gene function and maintenance of R gene diversity within a plant species are therefore of great interest. In the Arabidopsis accession Wassilewskija, the RPP1 region on chromosome 3 contains four genetically linked recognition specificities, conditioning resistance to different isolates of the biotrophic oomycete Peronospora parasitica (downy mildew). We show that three of four tightly linked genes in this region, designated RPP1-WsA, RPP1-WsB, and RPP1-WsC, encode functional products of the NBS-LRR (nucleotide binding site-leucine-rich repeat) R protein class. They possess a TIR Toll, interleukin-1, resistance domain that is characteristic of certain other NBS-LRR-type R proteins, but in addition, they have unique hydrophilic or hydrophobic N termini. Together, the three RPP1 genes account for the spectrum of resistance previously assigned to the RPP1 region and thus comprise a complex R locus. The distinct but partially overlapping resistance capabilities conferred by these genes are best explained by the hypothesis that each recognizes a different pathogen avirulence determinant. We present evidence suggesting that the RPP genes at this locus are subject to the same selective forces that have been demonstrated for structurally different LRR-type R genes