Expression Pattern Implicates a Potential Role for Luman Recruitment Factor in the Process of Implantation in Uteri and Development of Preimplantation Embryos in Mice

• Published in: Journal of Reproduction and Development Vol. 59; no. 3; pp. 245 - 251
• Main Authors: YANG, Yanzhou, JIN, Yaping, MARTYN, Amanda C, LIN, Pengfei, SONG, Yujie, CHEN, Fenglei, HU, Linyong, CUI, Chenchen, LI, Xiao, LI, Qian, LU, Ray, WANG, Aihua
• Format: Journal Article
• Language: English
• Published: Japan THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT 2013; The Society for Reproduction and Development
• Subjects: Animals; Blastocyst - metabolism; Cyclic AMP Response Element-Binding Protein - metabolism; Decidua - metabolism; DNA-Binding Proteins - metabolism; Embryo Implantation - physiology; Estradiol; Estradiol - metabolism; Female; Gene Expression Regulation, Developmental; Implantation; Luman recruitment factor LRF/CREBRF; Mice; Original; Pregnancy; Pregnancy, Animal; Progesterone; Progesterone - metabolism; Real-Time Polymerase Chain Reaction; Signal Transduction; Steroids - metabolism; Time Factors; Transcription Factors - metabolism; Uteri; Uterus - physiology
• ISSN: 0916-8818; 1348-4400
• DOI: 10.1262/jrd.2012-137

Luman/CREB3 recruitment factor (LRF or CREBRF) was identified as a regulator of Luman (or CREB3) that is involved in the unfolded protein response during endoplasmic reticulum stress. Luman is implicated in a multitude of functions ranging from viral infection and immunity to cancer. The biological function of LRF, however, is unknown. In this paper, we report that uteri of pregnant mice and embryos displayed enhanced LRF expression at all stages, and the expressed LRF was found to be localized specifically at implantation sites. On the other hand, uteri of mice induced for delayed implantation or pseudopregnant mice showed low levels of LRF expression, suggesting that LRF mediates uterine receptivity during implantation. Further, expression of LRF was found to be modulated by steroid hormones such as progesterone and estradiol. This study thereby identifies a potential role for LRF in the process of implantation in uteri and development of preimplantation embryos in mice.