Hippocampal subfields revealed through unfolding and unsupervised clustering of laminar and morphological features in 3D BigBrain

• Published in: NeuroImage (Orlando, Fla.) Vol. 206; p. 116328
• Main Authors: DeKraker, J., Lau, J.C., Ferko, K.M., Khan, A.R., Köhler, S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2020; Elsevier Limited; Elsevier
• Subjects: CA1 Region, Hippocampal - anatomy & histology; CA2 Region, Hippocampal - anatomy & histology; CA3 Region, Hippocampal - anatomy & histology; Cluster Analysis; Computational neuroscience; Cortical folding; Cortical unfolding; Datasets; Datasets as Topic; Dentate Gyrus - anatomy & histology; Hippocampus; Hippocampus - anatomy & histology; Histology; Humans; Imaging, Three-Dimensional; Magnetic resonance imaging; Models, Anatomic; Morphology; Neocortex; Neuroimaging; Principal Component Analysis; Subfields; Unsupervised Machine Learning; Subfields; Histology; Cortical unfolding; Cortical folding; Morphology; Hippocampus
• ISSN: 1053-8119; 1095-9572; 1095-9572
• DOI: 10.1016/j.neuroimage.2019.116328

The internal structure of the human hippocampus is challenging to map using histology or neuroimaging due to its complex archicortical folding. Here, we aimed to overcome this challenge using a unique combination of three methods. First, we leveraged a histological dataset with unprecedented 3D coverage, BigBrain. Second, we imposed a computational unfolding framework that respects the topological continuity of hippocampal subfields, which are traditionally defined by laminar composition. Third, we adapted neocortical parcellation techniques to map the hippocampus with respect to not only laminar but also morphological features. Unsupervised clustering of these features revealed subdivisions that closely resemble gold standard manual subfield segmentations. Critically, we also show that morphological features alone are sufficient to derive most hippocampal subfield boundaries. Moreover, some features showed differences within subfields along the hippocampal longitudinal axis. Our findings highlight new characteristics of internal hippocampal structure, and offer new avenues for its characterization with in-vivo neuroimaging. •The human hippocampus was investigated quantitatively in 3D BigBrain histology.•Morphological and laminar features were mapped to the unfolded hippocampus.•Unsupervised clustering of these features revealed hippocampal subfields.•Morphological features alone were sufficient to delineate subfield boundaries.•Gyrifications were seen throughout the head, body, and tail of subfield CA1.