Comprehensive measurements of hydroxylinoleate and hydroxyarachidonate isomers in blood samples from primary open-angle glaucoma patients and controls

• Published in: Scientific reports Vol. 9; no. 1; p. 2171
• Main Authors: Umeno, Aya, Tanito, Masaki, Kaidzu, Sachiko, Takai, Yasuyuki, Horie, Masanori, Yoshida, Yasukazu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.02.2019; Nature Publishing Group
• Subjects: 692/308/53/2421; 692/53/2421; Antioxidants; Arachidonic acid; Glaucoma; Humanities and Social Sciences; Isomers; Linoleic acid; multidisciplinary; Oxidation; Oxidation process; Oxygen; Science; Science (multidisciplinary); Serum levels
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-36952-6

We previously reported that lower systemic antioxidant capacity is involved in primary open-angle glaucoma (POAG) and exfoliation syndrome pathogeneses as measured by ferric-reducing activity. In the present study, we measured hydroxylinoleate (HODE) and hydroxyarachidonate (HETE) isomer serum levels after sample reduction and saponification to investigate POAG pathogenesis. POAG patients (n = 198) were recruited and divided into normal- and high-tension glaucoma groups (n = 84 and 114, respectively) depending on intraocular pressure. Total HODE (/linoleic acid) and HETE (/arachidonic acid) serum levels were significantly higher in the POAG group (211.9 ± 143.0 and 181.0 ± 164.1 µmol/mol, respectively) than in controls (167.1 ± 105.2 and 132.5 ± 139.7 µmol/mol, p = 0.0025 and 0.0101, respectively). The associations between HODEs/HETEs and glaucoma were further confirmed by multivariate analyses after adjusting for differences in demographic parameters. Among the HODE isomers, the levels of 9- and 13-(Z,E)-HODEs (p = 0.0014) and singlet oxygen-specific products (i.e., 10- and 12-(Z,E)-HODEs, p = 0.0345) were higher in the POAG group than in controls, while free radical-mediated oxidation-specific products (i.e., 9- and 13-(E,E)-HODEs, p = 0.0557) demonstrated a marginal difference. Enzymatic and singlet oxygen-mediated fatty acid oxidation may be major pathways of oxidation process in glaucoma subjects.