Facile preparation of organic-inorganic hybrid polymeric ionic liquid monolithic column with a one-pot process for protein separation in capillary electrochromatography

• Published in: Analytical and bioanalytical chemistry Vol. 406; no. 28; pp. 7175 - 7183
• Main Authors: Liu, Cuicui, Deng, Qiliang, Fang, Guozhen, Feng, Xue, Qian, Hailong, Wang, Shuo
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.11.2014; Springer; Springer Nature B.V
• Subjects: Alkylbenzenes; Analytical Chemistry; Aniline; Animals; Biochemistry; Biocompatibility; Capillarity; Capillary Electrochromatography - methods; Catalysts; Characterization and Evaluation of Materials; Chemical properties; Chemistry; Chemistry and Materials Science; Chickens; Columns (process); Copolymerization; Egg White - chemistry; Electrochromatography; Food Science; Formic acid; Fourier analysis; Fourier transforms; Infrared spectra; Inorganic Chemicals - chemistry; Ionic Liquids; Ions; Laboratory Medicine; Microscopy, Electron, Scanning; Monitoring/Environmental Analysis; Organic Chemicals - chemistry; Polymers - chemistry; Protein purification; Proteins; Proteins - chemistry; Proteins - isolation & purification; Research Paper; Scanning electron microscopy; Separation; Silicon Dioxide - chemistry; Sol gel process; Sol-gel processes; China; Protein separation; Capillary electrochromatography; Non-hydrolytic sol-gel; Hybrid monolithic column
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-014-8137-5

An organic-inorganic hybrid monolithic column based on 1-vinyl-3-dodecylimidazolium bromide (VC 12 Im + Br − ) has been prepared in a single step by combining radical copolymerization with a non-hydrolytic sol-gel (NHSG) process. The NHSG process was significantly shortened to 6 h by using formic acid as catalyst. For comparison, we also prepared polymeric ionic liquid (PIL) monolithic columns by hydrolytic sol-gel and organic polymeric process, respectively. The resulting monolithic columns were characterized by Fourier transform infrared spectra, scanning electron microscopy, and Brunauer-Emmett-Teller. Under the capillary electrochromatography mode, these columns were applied to separate alkylbenzenes, anilines, and proteins, respectively. The results indicated that the NHSG-based hybrid PIL monolithic column exhibited the highest column efficiency among the three types of columns; organic solvent, commonly required by the traditional columns to achieve satisfactory separation efficiency for proteins, was absent in the NHSG-based hybrid PIL monolithic column because of the biocompatibility of the VC 12 Im + Br − , which was beneficial to analysis of protein containing samples. In order to demonstrate its application potential, the developed NHSG-based hybrid PIL monolithic column was also employed to separate egg white sample.