Identification of ultraviolet B radiation-induced microRNAs in normal human dermal papilla cells

• Published in: Molecular medicine reports Vol. 10; no. 4; pp. 1663 - 1670
• Main Authors: CHA, HWA JUN, KIM, OK-YEON, LEE, GANG TAI, LEE, KWANG SIK, LEE, JAE HO, PARK, IN-CHUL, LEE, SU-JAE, KIM, YU RI, AHN, KYU JOONG, AN, IN-SOOK, AN, SUNGKWAN, BAE, SEUNGHEE
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.10.2014; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Analysis; Apoptosis; Apoptosis - radiation effects; Cell cycle; Cell Cycle Checkpoints - radiation effects; Cell death; Cell survival; Cell Survival - radiation effects; Cells; Cells, Cultured; Cytotoxicity; Dermis - cytology; Dermis - radiation effects; DNA damage; DNA microarrays; Down-Regulation - radiation effects; Fibroblasts; Gene expression; Genes; Genetic aspects; Hair; Health aspects; human dermal papilla cells; Humans; Hybridization; Keratinocytes; Melanocytes; microarray; MicroRNA; MicroRNAs; MicroRNAs - metabolism; miRNA; Physiological aspects; Reactive oxygen species; Reactive Oxygen Species - metabolism; Skin; Software; Studies; ultraviolet B; Ultraviolet radiation; Ultraviolet Rays; Up-Regulation - radiation effects; South Korea; United States > US
• ISSN: 1791-2997; 1791-3004
• DOI: 10.3892/mmr.2014.2418

Ultraviolet (UV) radiation impairs intracellular functions by directly damaging DNA and by indirectly generating reactive oxygen species (ROS), which induce cell cycle arrest and apoptosis. UV radiation can also alter gene expression profiles, including those of mRNA and microRNA (miRNA). The effects of UV radiation on cellular functions and gene expression have been widely documented in human skin cells such as keratinocytes, melanocytes and dermal fibroblasts, but the effect it has on other types of skin cell such as dermal papilla cells, which are crucial in the induction of hair follicle growth, remains unknown. In the current study, the effect of UV radiation on physiological changes and miRNA-based expression profiles in normal human dermal papilla cells (nHDPs) was investigated. UVB radiation of ≥50 mJ/cm2 displayed high cytotoxicity and apoptosis in a dose-dependent manner. In addition, ROS generation was exhibited in UVB-irradiated nHDPs. Furthermore, using miRNA microarray analysis, it was demonstrated that the expression profiles of 42 miRNAs in UVB-irradiated nHDPs were significantly altered compared with those in the controls (35 upregulated and 7 downregulated). The biological functions of the differentially expressed miRNAs were studied with gene ontology analysis to identify their putative target mRNAs, and were demonstrated to be involved in cell survival- and death-related functions. Overall, the results of the present study provide evidence that miRNA-based cellular mechanisms may be involved in the UVB-induced cellular response in nHDPs.