Isolation of extracellular vesicles: Determining the correct approach (Review)

• Published in: International journal of molecular medicine Vol. 36; no. 1; pp. 11 - 17
• Main Authors: SZATANEK, RAFAL, BARAN, JAREK, SIEDLAR, MACIEJ, BAJ-KRZYWORZEKA, MONIKA
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.07.2015; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Adsorption; Biological Transport; Body Fluids - cytology; Cell Communication; Cell culture; Cell Culture Techniques - methods; Cell interaction; Cell organelles; Cell research; Cell-Derived Microparticles - physiology; Chromatography, Gel; Culture Media, Conditioned; Cytokines; differential centrifugation; Enzymes; exosomes; Exosomes - physiology; Extracellular vesicles; Humans; Kinases; microvesicles; Properties; Proteins; Studies; Ultracentrifugation; Urine

The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/traffi...