Isolation of extracellular vesicles: Determining the correct approach (Review)

The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/traffi...

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Published inInternational journal of molecular medicine Vol. 36; no. 1; pp. 11 - 17
Main Authors SZATANEK, RAFAL, BARAN, JAREK, SIEDLAR, MACIEJ, BAJ-KRZYWORZEKA, MONIKA
Format Journal Article
LanguageEnglish
Published Greece D.A. Spandidos 01.07.2015
Spandidos Publications
Spandidos Publications UK Ltd
Subjects
Adsorption
Biological Transport
Body Fluids - cytology
Cell Communication
Cell culture
Cell Culture Techniques - methods
Cell interaction
Cell organelles
Cell research
Cell-Derived Microparticles - physiology
Chromatography, Gel
Culture Media, Conditioned
Cytokines
differential centrifugation
Enzymes
exosomes
Exosomes - physiology
Extracellular vesicles
Humans
Kinases
microvesicles
Properties
Proteins
Studies
Ultracentrifugation
Urine
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Summary:The discovery of extracellular vesicles (EVs) has revised the interpretation of intercellular communication. It is now well established that EVs play a significant role in coagulation, inflammation, cancer and stem cell renewal and expansion. Their release presents an intriguing, transporting/trafficking network of biologically active molecules, which are able to reach and modulate the function/behavior of the target cells in a variety of ways. Moreover, the presence of EVs in various body fluids points to their potential for use as biomarkers and prognostic indicators in the surveillance/monitoring of a variety of diseases. Although vast knowledge on the subject of EVs has accumulated over the years, there are still fundamental issues associated with the correct approach for their isolation. This review comprises the knowledge on EV isolation techniques that are currently available. The aim of this review was to make both experienced researchers and newcomers to the field aware that different types of EVs require unique isolation approaches. The realization of this 'uniqueness' is the first step in the right direction for the complete assessment of EVs.
Bibliography:ObjectType-Article-1
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ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2015.2194