Plasma membrane inositol 1,4,5-Trisphosphate-Activated channels mediate signal transduction in lobster olfactory receptor neurons

• Published in: Neuron (Cambridge, Mass.) Vol. 9; no. 5; pp. 907 - 918
• Main Authors: Fadool, D.A., Ache, B.W.
• Format: Journal Article
• Language: English
• Published: Cambridge, MA Elsevier Inc 01.11.1992; Cell Press
• Subjects: Animals; Antibodies; Biochemistry. Physiology. Immunology; Biological and medical sciences; Calcium Channels; Cell Membrane - physiology; Crustacea; Electric Conductivity; Fundamental and applied biological sciences. Psychology; Inositol 1,4,5-Trisphosphate - pharmacology; Inositol 1,4,5-Trisphosphate Receptors; Invertebrates; Ion Channels - drug effects; Ion Channels - physiology; Mice; Nephropidae - physiology; Neurons - physiology; Odorants; Physiology. Development; Receptors, Cell Surface - immunology; Receptors, Cytoplasmic and Nuclear; Second Messenger Systems - physiology; Sensory Receptor Cells - physiology; Signal Transduction - physiology; Smell - physiology; Cell culture; Phosphoinositide; Panulirus argus; Electrophysiology; Patch clamp method; Ionic channel; Olfactory receptor; Sensory receptor; Ionic current; Macrura; Crustacea; Marine environment; Olfactive system; Signal transduction; Arthropoda; Second messenger; Decapoda; Invertebrata; Odor
• ISSN: 0896-6273; 1097-4199
• DOI: 10.1016/0896-6273(92)90243-7

Inositol 1,4,5-trisphosphate (IP 3) selectively evokes an inward (excitatory) current in cultured lobster olfactory receptor neurons (ORNs) and directly activates two types of channels in cell-free patches of plasma membrane from the ORNs. The IP 3-activated channels have kinetic properties of odor-activated channels in the ORNs and pharmacological properties of intracellular IP 3-activated channels in other systems. An antibody directed against an intracellular, cerebellar IP 3 receptor recognizes a protein with a molecular weight similar to the mammalian receptor in the ORNs. The antibody selectively increases odor-evoked inward currents and IP 3-activated unitary currents in the ORNs. The data provide further evidence for IP 3 as an olfactory second messenger and implicate at least one and possibly two novel plasma membrane I P, receptors in olfactory transduction.