Lateral intracerebroventricular injection of Apelin- 13 inhibits apoptosis after cerebral ischemia/reperfusion injury

• Published in: Neural regeneration research Vol. 10; no. 5; pp. 766 - 771
• Main Authors: Yan, Xiao-Ge, Cheng, Bao-Hua, Wang, Xin, Ding, Liang-Cai, Liu, Hai-Qing, Chen, Jing, Bai, Bo
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01.05.2015; Medknow Publications & Media Pvt. Ltd; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: active zone stability; Animal research models; apelin; Apelin-13; apoplexy; Apoptosis; axon pathfinding; axonal regeneration; Bcl-2; biomaterial; biomaterials; Brain; brain activity; brain injury; Carotid arteries; caspase-3; cell differentiation; cell migration; cell transplant; cell transplantation; central nervous system; Cerebral ischemia; cerebral ischemia/reperfusion injury; cerebral subarachnoid hemorrhage; cognition; dephosphorylation; Diagnosis; Drosophila; exosome; extracellular vesicle; functional magnetic resonance imaging; Genetic aspects; ginsenoside Rg1; GSK-3ß; healthy; hemodynamic phases; hypoxic-ischemic brain damage; injury; Ischemia; lateral intracerebroventricular injection; Liprin-α; living scaffolds; metabolic crises; microglia; microRNA; microvesicle; nanotechnology; nerve apoptosis; nerve gap; nerve reconstruction; nerve regeneration; neural progenitor; neural regeneration; neural stem cells; neural tissue engineering; neurite outgrowth; neurodegeneration; neurodegenerative disease; neuroimaging; neuromuscular junction; neuron; neuroprotection; neuroregeneration; neurotrauma; NSFC grants; Ostomy; participants; peripheral nerve injury; Physiology; plasticity; PP2A; regeneration; regenerative medicine; regional homogeneity; repair; Risk factors; Rodents; Schwann cell; spinal cord injury; stem cell; subacute; Syd-1; therapy; tissue engineering; Veins & arteries; volunteers; 侧脑室注射; 免疫组织化学染色; 神经细胞凋亡; 缺血/再灌注损伤; 缺血再灌注损伤; China; Beijing China; United States > US; Bcl-2; cerebral ischemia/reperfusion injury; brain injury; neural regeneration; neuroprotection; NSFC grants; Apelin-13; lateral intracerebroventricular injection; nerve apoptosis; nerve regeneration; caspase-3
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.157243

Apelin- 13 inhibits neuronal apoptosis caused by hydrogen peroxide, yet apoptosis following cerebral ischemia-reperfusion injury has rarely been studied. In this study, Apelin-13 （0.1 μg/g） was injected into the lateral ventricle of middle cerebral artery occlusion model rats. TTC, TUNEL, and immuno- histochemical staining showed that compared with the cerebral ischemia/reperfusion group, infarct volume and apoptotic cell number at the ischemic penumbra region were decreased in the Apelin-13 treatment group. Additionally, Apelin-13 treatment increased Bcl-2 immtmoreactivity and decreased caspase-3 immunoreactivity, Our findings suggest that Apelin-13 is neuroprotective against cerebral ischemia/reperfusion injury through inhibition of neuronal apoptosis.