In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

• Published in: AMB Express Vol. 11; no. 1; p. 109
• Main Authors: Sakr, Masarra M., Elkhatib, Walid F., Aboshanab, Khaled M., Mantawy, Eman M., Yassien, Mahmoud A., Hassouna, Nadia A.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 27.07.2021; Springer Nature B.V; SpringerOpen
• Subjects: Animal models; Biomedical and Life Sciences; Biotechnology; Burn infection; Evaluation; Healing; Hydrogels; In vivo methods and tests; Infections; Life Sciences; Microbial Genetics and Genomics; Microbiology; Mortality; Multidrug resistance; N-acylhomoserine lactonase; Original; Original Article; P. aeruginosa; Pseudomonas aeruginosa; Quorum quenching; Stability; Survival; Thermal injury; Virulence; Burn infection; Quorum quenching; N-acylhomoserine lactonase
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-021-01269-7

Failure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa , a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.