Conduction-Band-Edge Ionization Thresholds of DNA Components in Aqueous Solution

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 95; no. 10; pp. 5550 - 5555
• Main Authors: Fernando, Harshica, Papadantonakis, George A., Kim, Nancy S., LeBreton, Pierre R.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 12.05.1998; National Acad Sciences; National Academy of Sciences; The National Academy of Sciences
• Subjects: Biochemistry; Biological Sciences; Chemical Phenomena; Chemistry, Physical; Deoxycytidine Monophosphate - chemistry; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA Damage; Electrons; Energy; Experiments; Indoles; Ionization; Ionization potentials; Ions; Models, Chemical; Molecular orbitals; Nucleotides; Orbitals; Phosphates; Photochemistry; Solutions; Sugars; Thymidine Monophosphate - chemistry
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.95.10.5550

Numerous investigations have focused on DNA damage induced by ionizing radiation; however, photoionization threshold energies of nucleic acid components in aqueous solution are not known. Herein, data from gas-phase photoelectron experiments have been combined with results from self-consistent field and post-self-consistent field molecular orbital calculations and with theoretical Gibbs free energies of hydration to describe aqueous ionization energies of 2′-deoxythymidine 5′-phosphate (5′-dTMP-) and 2′-deoxycytidine 5′-phosphate (5′-dCMP-). For the test molecules, indole and tryptophan, this approach yields aqueous ionization energies (4.46 and 4.58 eV, respectively) in agreement with experimental values (4.35 and 4.45 eV). When uridine and 2′-deoxythymidine ionization energies are evaluated, the results agree with recent data from 193-nm laser measurements indicating that uridine ionization occurs via a one-photon event. For 5′-dCMP-and 5′-dTMP-, a comparison of aqueous ionization energies with gas-phase ionization potentials (IPs) indicates that hydration alters the relative energies of ionization events. In the gas phase, phosphate vertical IPs are ∼ 1.3 eV smaller than base IPs. In aqueous solution, the base and phosphate ionization energies are more similar, and only differ by ∼ 0.5 eV. For 5′-dCMP-and 5′-dTMP-, the increased favorableness of base ionization, which accompanies hydration, is consistent with experimental data indicating that, at 77 K in aqueous perchlorate glasses, the primary photoionization pathway involves base ionization followed by deprotonation.