Influences of protein ingestion on glucagon‐like peptide (GLP)‐1‐immunoreactive endocrine cells in the chicken ileum

• Published in: Animal science journal Vol. 85; no. 5; pp. 581 - 587
• Main Authors: Monir, Mohammad M, Hiramatsu, Kohzy, Matsumoto, Sachiko, Nishimura, Kei, Takemoto, Chihiro, Shioji, Tomomi, Watanabe, Takafumi, Kita, Kazumi, Yonekura, Shinichi, Roh, Sang Gun
• Format: Journal Article
• Language: English
• Published: Australia Blackwell 01.05.2014; Blackwell Publishing Ltd
• Subjects: Animals; chicken; chickens; Chickens - anatomy & histology; Chickens - immunology; crude protein; Diet; dietary protein; Dietary Proteins - pharmacology; Digestive system; Enteroendocrine Cells - cytology; Enteroendocrine Cells - immunology; experimental diets; feed intake; Glucagon-Like Peptide 1; ileum; Ileum - cytology; Ileum - immunology; Immunohistochemistry; ingestion; intestine; Male; Peptides; Poultry; protein ingestion; protein intake; Proteins; regression analysis; weight gain; Zoology; chicken; intestine; immunohistochemistry; protein ingestion; glucagon-like peptide-1
• ISSN: 1344-3941; 1740-0929
• DOI: 10.1111/asj.12177

Influences of a specific dietary nutrient on glucagon‐like peptide (GLP)‐1‐containing cells in the chicken intestine are not yet clear. Significance of dietary protein level on GLP‐1‐containing cells in the chicken ileum was investigated. Chickens fed control or experimental diets of varying protein levels were examined using immunohistochemical and morphometrical techniques. We show that the protein ingestion had an impact on the activities of GLP‐1‐immunoreactive cells in the chicken ileum. Weight gains declined with decreasing dietary crude protein (CP) levels, but no significant differences were detected in the daily feed intake and villous height. GLP‐1‐immunoreactive cells with a round or oval shape were frequently observed in the lower CP level groups (4.5% and 0%). Frequencies of occurrence of GLP‐1‐immunoreactive cells were 41.1 ± 4.1, 38.5 ± 4, 34.8 ± 3.1 and 34.3 ± 3.7 (cells/mm², mean ± SD) for dietary CP level of 18%, 9%, 4.5% and 0% groups, respectively and significant differences were recognized between the control and lower CP level groups (P < 0.05). Multiple regression analysis indicated a significant correlation between the daily protein intake and frequencies of occurrence of GLP‐1‐immunoreactive cells. The protein ingestion is one of the signals that influence GLP‐1‐containing cells in the chicken small intestine.