重组大肠杆菌发酵表达菊酯类农药降解酶培养基优化

通过单因素及正交试验,研究了碳源、有机氮源、无机氮源对重组大肠杆菌Eschericha.coli BL21 （DE3）/pET-28a-est825菌体生长及产酶的影响,进而优化了产酶培养基,确定最佳培养基组成为：甘油20 g/L,酵母粉20g/L,硫酸铵5 g/L,NaCl 5 g/L,Na2HP04·12H2O 10.7 g/L,KH2PO42.7g/L,硫酸卡那霉素50 μg/L,pH 7.0.采用此培养基发酵所得发酵液酶活力较优化前提高了86％....

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Bibliographic Details
Published in广东农业科学 Vol. 41; no. 5; pp. 134 - 137
Main Author 黄皓 梁卫驱 罗华建 胡珊 李艳芳 陈仕丽 徐匆
Format Journal Article
LanguageChinese
Published 东莞市农业科学研究中心,广东东莞,523086 2014
Subjects
优化
培养基
菊酯类农药降解酶
重组大肠杆菌
重组大肠杆菌
培养基
菊酯类农药降解酶
优化
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ISSN1004-874X

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Summary:通过单因素及正交试验,研究了碳源、有机氮源、无机氮源对重组大肠杆菌Eschericha.coli BL21 （DE3）/pET-28a-est825菌体生长及产酶的影响,进而优化了产酶培养基,确定最佳培养基组成为：甘油20 g/L,酵母粉20g/L,硫酸铵5 g/L,NaCl 5 g/L,Na2HP04·12H2O 10.7 g/L,KH2PO42.7g/L,硫酸卡那霉素50 μg/L,pH 7.0.采用此培养基发酵所得发酵液酶活力较优化前提高了86％.
Bibliography:44-1267/S
The medium for pyrethroid pesticide degradative enzyme expression by recombinant Escherichia coli was optimized by investigating the effcets of carbon sources, organic nitrogen sources and inorganic nitrogen sources on cell growth and expression of degradative enzyme through single facor and orthogonal experiments. The results showed that the optimal medium composition was glycerol 20 g/L, yeast extract 20 g/L, （NN4）2S04 5 g/L, NaCl 5 g/L,Na2HP04·12H2O 10.7 g/L,KH2PO42.7g/L, KH2P04 2.7 g/L, kanamycin sulfate 50 μg/L,pH 7.0. By using the optimum medium, the activity of degradative enzyme incrcasedhy about 86% compared with the original medium.
HUANG Hao LIANG Wei-qu LUO Hua-jian HU Shan LI Yan-fang CHEN Shi-li XU Cong （Dongguan Agricultural Research Center, Dongguan 523086, China）
pyrethroid pesticide degradative enzyme; recombinant Escherichia toll; medium; optimization
ISSN:1004-874X