大麻多倍体诱导的初步研究

利用秋水仙素对大麻进行多倍体种质的诱导,获得大麻多倍体种质,以期提高大麻的药效价值或改良其他品质性状。以大麻种子为材料,在不同浓度秋水仙素溶液中处理不同时间,并对所得材料的倍性利用进行形态及细胞学鉴定。结果表明,0.2%和0.15%的秋水仙碱分别处理24 h和36 h,可获得较理想的诱变效果,经秋水仙素处理后,大麻细胞发生了明显的加倍,但被诱导植株仍然处于嵌合状态。...

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Published in江西农业学报 Vol. 25; no. 6; pp. 51 - 54
Main Author 田斌 郭鸿彦 杨明 辛培尧
Format Journal Article
LanguageChinese
Published 西南林业大学 国家林业局西南地区生物多样性保育重点实验室,云南 昆明,650224%云南省农业科学院 经济作物研究所,云南 昆明,650201 2013
Subjects
多倍体
大麻
秋水仙素
种子
Cannabis sativa
Seed
多倍体
Colchicine
种子
秋水仙素
大麻
Polyploid
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ISSN1001-8581

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Summary:利用秋水仙素对大麻进行多倍体种质的诱导,获得大麻多倍体种质,以期提高大麻的药效价值或改良其他品质性状。以大麻种子为材料,在不同浓度秋水仙素溶液中处理不同时间,并对所得材料的倍性利用进行形态及细胞学鉴定。结果表明,0.2%和0.15%的秋水仙碱分别处理24 h和36 h,可获得较理想的诱变效果,经秋水仙素处理后,大麻细胞发生了明显的加倍,但被诱导植株仍然处于嵌合状态。
Bibliography:36-1124/S
Cannabis sativa ; Seed; Polyploid; Cotchicine
TIAN Bin, GUO Hong- yan , YANG Ming , XIN Pei -yao （ 1. Key Laboratory of Biodiversity Conservation in Southwestern China, State Forestry Administration, Southwest Forestry Uni- versity, Kunming 650224, China; 2. Institute of Economic Crop, Yunnan Academy of Agricultural Sciences, Kunming 650201, China）
In order to improve the medicinal value and other quality characters of Cannabis sativa, we used colchicine to induce its polyploid germplasms. The seeds of Cannabis sativa were treated with different concentrations of colchicine for various times. The acquired polyploidy was identified by the methods of morphology and cytology. The results showed that the better polyploid inducement effect could be obtained by treating the seeds with 0.20% （or 0.15% ） colchicine for 24 h （or 36 h）. The polyploid cells of Cannabis sativa were found obviously after treatment of colchicine, but the treated plants were still in chimeric status.
ISSN:1001-8581