实验性蛛网膜下腔出血后脑动脉的基因表达谱及功能分析

目的探讨兔蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）基底动脉和正常基底动脉基因表达的差异。方法兔SAH模型脑基底动脉及正常兔脑基底动脉的cDNA基因芯片下载于GEO数据库。使用Bioconductor软件对芯片进行分析筛选，并用Cytoscape软件对差异表达基因进行功能富集和信号通路分析。随后选取成年雄性日本大耳兔6只，随机分为正常对照组（n=3）和SAH模型组（n=3）。采用枕大池二次注血法复制兔SAH模型，取第0天未行手术处理的对照兔基底动脉标本RNA和第5天SAH后基底动脉标本RNA行qRT-PCR，验证部分差异基因。结果在兔正常基底动脉和兔SAH模型基底动脉中共获得4356个差异...

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Bibliographic Details
Published in天津医药 Vol. 45; no. 4; pp. 355 - 358
Main Author 甘宁;潘勤;刘思思;任可;周帅;董海青;宋朝彦;王毅
Format Journal Article
LanguageChinese
Published 保定市第一中心医院总院神经外科 071000%吉林大学药学院%天津医科大学总医院神经外科,天津市神经病学研究所 2017
Subjects
蛛网膜下腔出血;血管痉挛,颅内;基底动脉;寡核苷酸序列分析;GO分析;通路分析
basilar artery
GO analysis
寡核苷酸序列分析
intracranial
vasospasm
pathway analysis
血管痉挛,颅内
蛛网膜下腔出血
subarachnoid hemorrhage
基底动脉
oligonucleotide array sequence analysis
GO分析
通路分析
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ISSN0253-9896
DOI10.11958/20170062

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Summary:目的探讨兔蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）基底动脉和正常基底动脉基因表达的差异。方法兔SAH模型脑基底动脉及正常兔脑基底动脉的cDNA基因芯片下载于GEO数据库。使用Bioconductor软件对芯片进行分析筛选，并用Cytoscape软件对差异表达基因进行功能富集和信号通路分析。随后选取成年雄性日本大耳兔6只，随机分为正常对照组（n=3）和SAH模型组（n=3）。采用枕大池二次注血法复制兔SAH模型，取第0天未行手术处理的对照兔基底动脉标本RNA和第5天SAH后基底动脉标本RNA行qRT-PCR，验证部分差异基因。结果在兔正常基底动脉和兔SAH模型基底动脉中共获得4356个差异表达的基因，其中920个差异表达基因（P＜0.05），如GRIK1、MYH13、ZNF45、SAA3、RLN1、MSR1等。功能富集分析结果显示差异表达基因涉及钙离子跨膜转运蛋白活性调节、离子跨膜转运负调控、钾离子转运调控、JAK-STAT信号通路级联的正调节等相关生物学过程。通路分析显示这些基因与钙信号通路、cGMP-PKG信号通路、HIF-1信号通路、PI3K-Akt信号通路等有关。qRT-PCR验证表明SAH模型组MSR1下调，与芯片结果一致。结论兔造模后CVS基底动脉中存在基因的差异化表达，并且MSR1基因可以作为研究CVS病理机制的潜在靶点。
Bibliography:subarachnoid hemorrhage; vasospasm, intracranial; basilar artery; oligonucleotide array sequence analysis;GO analysis;pathway analysis
Objective To explore the difference of gene expression profiling between normal basilar arteries and basilar arteries of cerebral vasospasm(CVS)after subarachnoid hemorrhage(SAH)in rabbits.Methods cDNA chip of normal basilar arteries and basilar arteries of CVS after SAH in rabbits were downloaded from GEO database.The chip was analyzed and screened by Bioconductor software,and function enrichment and pathway analysis of the differentially expressed genes were analyzed by Cytoscape software.Then6adult male Japanese rabbits were used,and randomly divided into normal control group(n=3)and SAH model group(n=3).Rabbit SAH models were established by cisterna secondaryblood-injection method.RNA data of normal basilar artery specimens on the0day and basilar artery specimens after SAH on the5-day were used to validate the parts of differentially expressed genes by qRT-PCR.Results A tot
ISSN:0253-9896
DOI:10.11958/20170062