Characterization of proteins Msp22 and Msp75 as vaccine antigens of Moraxella catarrhalis

• Published in: Vaccine Vol. 27; no. 50; pp. 7065 - 7072
• Main Authors: Ruckdeschel, Elizabeth A, Brauer, Aimee L, Johnson, Antoinette, Murphy, Timothy F
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 23.11.2009; Elsevier
• Subjects: Allergy and Immunology; Animal models; Animals; Antibodies, Bacterial - blood; Antigens, Bacterial - immunology; Applied microbiology; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Immunization; Mice; Mice, Inbred BALB C; Microbiology; Miscellaneous; Moraxella (Branhamella) catarrhalis - immunology; Moraxella catarrhalis; Moraxellaceae Infections - immunology; Moraxellaceae Infections - prevention & control; Mouse pulmonary clearance; Obstructive lung disease; Otitis media; Rabbits; Recombinant Proteins - immunology; Vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Moraxella catarrhalis; Otitis media; Immunization; Vaccine; Mouse pulmonary clearance; Obstructive lung disease; Pseudomonadales; Lung disease; Respiratory disease; Middle ear disease; Rodentia; Clearance; Protein; Antigen; Vertebrata; Mammalia; Mouse; Bacteria; ENT disease; Bronchus disease; Obstructive pulmonary disease; Moraxellaceae
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2009.09.062

Abstract Moraxella catarrhalis is a respiratory tract pathogen causing otitis media in children and respiratory tract infections in adults with chronic obstructive pulmonary disease. This study examined two newly identified proteins as potential vaccine antigens. Antisera raised to recombinant purified proteins Msp22 and Msp75 recognized corresponding native proteins in multiple strains of M. catarrhalis . Vaccine formulations individually administered subcutaneously and intranasally showed enhanced clearance of M. catarrhalis in a mouse pulmonary clearance model by both routes of administration. Msp22 and Msp75 are antigenically conserved proteins that induce potentially protective immune responses and should be examined further as vaccine antigens for M. catarrhalis.