Ethanolamine plasmalogens derived from scallops stimulate both follicle-stimulating hormone and luteinizing hormone secretion by bovine gonadotrophs

• Published in: Scientific reports Vol. 12; no. 1; pp. 16789 - 12
• Main Authors: Kadokawa, Hiroya, Kotaniguchi, Miyako, Mawatari, Shiro, Saito, Risa, Fujino, Takehiko, Kitamura, Shinichi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 06.10.2022; Nature Publishing Group; Nature Portfolio
• Subjects: 631/601/1737; 692/163/2743; Alzheimer's disease; Brain research; Brain-derived neurotrophic factor; Cattle; Chromatography; Ethanolamine; Fatty acids; Follicle-stimulating hormone; Follicles; G protein-coupled receptors; Glycerol; Humanities and Social Sciences; Hypotheses; Infertility; Laboratory animals; Lipids; Liquid chromatography; Luteinizing hormone; Mass spectrometry; Mass spectroscopy; multidisciplinary; Neuroprotection; Pituitary; Pituitary (anterior); Pituitary gland; Process engineering; Science; Science (multidisciplinary); Scientific imaging; Secretion
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-022-20794-4

Brain ethanolamine plasmalogens (EPls) are the only known ligands of G-protein-coupled receptor 61, a novel receptor that stimulates follicle-stimulating hormone (FSH), but not luteinizing hormone (LH), secretion by bovine gonadotrophs. We hypothesized that the recently developed neuroprotective EPls extracted from scallop ( Pecten yessoensis ) (scallop EPls) could stimulate FSH secretion by gonadotrophs. To test this hypothesis, bovine gonadotrophs were cultured for 3.5 days and treated with increasing concentrations of scallop EPls. FSH secretion was stimulated by all tested concentrations of scallop EPls ( P  < 0.05). Surprisingly, LH secretion was stimulated by both 0.5 ( P  < 0.05) and 5 ( P  < 0.01) ng/mL of scallop EPls. To clarify the important differences between bovine brain and scallop EPls, we utilized two-dimensional liquid chromatography–mass spectrometry, which revealed 44 peaks, including 10 large peaks. Among them, eight were scallop-specific EPl molecular species, occupying approximately 58% of the total area percentage of scallop EPls. Almost all large peaks contained 4, 5, or 6 unsaturated double bonds in the carbon chain at the sn-2 position of the glycerol backbone. Our results showed that EPls from scallops, lacking pituitary glands, stimulated both FSH and LH secretion by bovine gonadotrophs.