Antifungal effect of tissue conditioners containing poly(acryloyloxyethyltrimethyl ammonium chloride)-grafted chitosan on Candida albicans growth in vitro

Denture stomatitis is a pathological condition affecting the mucosa underneath ill-fitting dentures, and Candida albicans is considered its main etiologic factor. Tissue conditioners are temporary lining materials often applied to dentures to treat inflamed tissues. However, tissue conditioners do n...

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Published inJournal of dental sciences Vol. 13; no. 2; pp. 160 - 166
Main Authors Lee, Hsin-Lin, Wang, Ren-Syue, Hsu, Yung-Chuang, Chuang, Chuan-Chung, Chan, Hui-Rong, Chiu, Hsien-Chung, Wang, Yi-Bing, Chen, Kuo-Yu, Fu, Earl
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.06.2018
Association for Dental Sciences of the Republic of China
Elsevier
Subjects
chitosan
denture stomatitis
Original
quaternization
tissue conditioner
chitosan
quaternization
tissue conditioner
denture stomatitis
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Summary:Denture stomatitis is a pathological condition affecting the mucosa underneath ill-fitting dentures, and Candida albicans is considered its main etiologic factor. Tissue conditioners are temporary lining materials often applied to dentures to treat inflamed tissues. However, tissue conditioners do not exert antifungal activity, and the soft surface texture harbors C. albicans easily. The aim of this study was to examine the antifungal activity of tissue conditioners modified with chitosan (CS) or a quaternized chitosan (QCS), which was synthesized by grafting 2-[(acryloyloxy)ethyl] trimethyl ammonium chloride onto CS. Tissue conditioners containing varying weight percentages of CS or QCS were prepared as experimental discs 10 mm in diameter and 1 mm in thickness. Samples were co-cultured with C. albicans and the number of colony forming units was recorded. Other evaluations included cell toxicity and tensile bond strength to the resin denture base. It was found significantly fewer fungal colonies in tissue conditioners modified with CS or QCS, and none when the weight percentage of QCS exceeded 5%. CS and QCS did not affect the viability of human gingival epithelium cells or fibroblasts, and tensile bond strength did not differ between control and modified tissue conditioners. This study provides a foundation for the development of QCS as a novel and safe antifungal agent applied to tissue conditioners in clinical practice.
ISSN:1991-7902
2213-8862
DOI:10.1016/j.jds.2017.06.004