Elevated levels of plasma VEGF in patients with dengue hemorrhagic fever

• Published in: FEMS immunology and medical microbiology Vol. 43; no. 1; pp. 99 - 102
• Main Authors: Tseng, Chien-Sen, Lo, Hoi-Wan, Teng, Hsiu-Chen, Lo, Wei-Chin, Ker, Chen-Guo
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 2005; Blackwell Publishing Ltd; Blackwell; Oxford University Press
• Subjects: Adult; Aged; Angiogenesis; Biological and medical sciences; Dengue; Dengue - blood; Dengue fever; Dengue hemorrhagic fever; Dengue virus; Dimers; Disease control; Endothelial cells; Enzyme-Linked Immunosorbent Assay; Female; Fibrin; Fibrin Fibrinogen Degradation Products - analysis; Fundamental and applied biological sciences. Psychology; Growth factors; Humans; Male; Microbiology; Middle Aged; Monocytes; Multiple regression analysis; Pathogenesis; Permeability; Physical growth; Plasma; Plasma levels; Severe Dengue - blood; Taiwan; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - blood; Vector-borne diseases; Viral diseases; Taiwan; Dengue hemorrhagic fever; Pathogenesis; Vascular endothelial growth factor; Dengue; Infection; Human; Hemorrhagic fever; Immunology; Microbiology; Viral disease; Arbovirus disease
• ISSN: 0928-8244; 1574-695X; 2049-632X
• DOI: 10.1016/j.femsim.2004.10.004

Vascular endothelial growth factor (VEGF) can be produced by monocytes and endothelial cells. It plays important role in angiogenesis and vascular permeability. The phenomenon of extensive plasma leakage into various serous cavities of the body is a cardinal symptom of dengue hemorrhagic fever (DHF). This study was performed to investigate the role of VEGF in patients with DHF. Plasma samples collected from the 53 dengue fever (DF) patients (including 14 patients with DHF), and 5 additional subjects with non-dengue febrile illness as controls were tested for VEGF levels using commercial enzyme-linked immunosorbent assay (ELISA) kits. The results showed that median plasma levels of VEGF in the patients with DHF (54.6 pg ml −1) were significantly higher than those of DF (14.6 pg ml −1) and control group (27.1 pg ml −1) ( P < 0.05). In addition, VEGF levels in DF patients were not significantly different from those of control patients with non-dengue febrile illness ( P = 0.17). Multiple regression analysis was used to analyze the clinical variables independently associated with VEGF levels. The data showed that D-dimer levels were significantly associated with VEGF levels. In this study, plasma VEGF levels in patients with DHF were significantly higher than values from DF patients. The association between increased plasma VEGF levels and increased plasma D-dimer levels in the patients with dengue illness suggests that activation of the fibrinolytic system may play a role in VEGF production in the patients with DF.